新型维甲酸衍生物ATPR对胶质瘤U87细胞增殖和分化影响.pdfVIP

684 　　肿瘤2013 年8 月第33 卷第8 期　TUM OR Vol. 33, August 2013 DOI: 10.3781/j .issn.1000-7431.2013.08.004 Copyright© 2013 by TUM OR Basic Research 基础研究 · 新型维甲酸衍生物ATPR对胶质瘤U87细胞增殖和分化的影响 方大钊，王伟杰，董　楠，付宪华，孙晓阳，丁涟沭 南京医科大学附属淮安第一医院神经外科，江苏淮安　223300 [摘要]　目的：探讨新型全反式维甲酸衍生物4- 氨基-2- 三氟甲基苯基维甲酸酯（4-amino-2-trifluoromethyl-phenyl retinate ，ATPR ）对人脑胶质瘤U87 细胞增殖及分化的影响。方法：不同浓度的ATPR 作用U87 细胞后，分别采 用细胞计数试剂盒-8 （cell counting kit-8，CCK-8 ）法及平板克隆形成实验检测其对细胞增殖的影响；光学显微 镜和电子显微镜下观察细胞形态的改变；FCM 法检测对细胞周期分布的影响；蛋白质印迹法检测分化指标胶质纤 维酸性蛋白 （glial fibrillary acidic protein ，GFAP ）的表达 。结果：ATPR 对U87 细胞的增殖抑制作用呈浓度相关 性 ，单细胞克隆形成率下降，细胞形态趋于成熟；FCM 法检测结果显示，G0/G1 期细胞所占比例增加、S 期细胞 所占比例减少，细胞呈G /G 期阻滞；蛋白质印迹法检测结果显示，GFAP 蛋白表达量上升 。结论：ATPR 对人 0 1 脑胶质瘤U87 细胞有一定增殖抑制作用并诱导其分化。 [关键词]　神经胶质瘤；维甲酸；细胞增殖；细胞分化；U87 细胞 [中图分类号]　R739.41 [文献标志码] A [文章编号]　1000-7431 (2013) 08-0684-06 Effects of a novel retinoic acid derivative ATPR on proliferation and differentiation of human glioma cell line U87 FANG Da-zhao, WANG Wei-jie, DONG Nan, FU Xian-hua, SUN Xiao-yang, DING Lian-shu Department of Neurosurgery, Huai’an First People’s Hospital, Nanjing Medical University, Huai’an 223300, Jiangsu Province, China [A BST RA CT]　Objective: To explore the effect of a novel retinoic acid derivative ATPR (4-amino-2- trifluoromethyl-phenyl retinate) on proliferation and differentiation of U87 glioma cells in vitro . Methods: After treatment with ATPR at different concentrations, the proliferation of U87 cells was evaluated by CCK-8 (cell counting kit-8) and colony formation assay. The change in morphology of U87 cells was observed under a light microscope and an electron microscope. The distribution of cell cycle was examined by flow cytomentry. The expression of gliom