压应力对组织工程软骨细胞增殖的影响.pdfVIP

窑2530 窑 南方医科大学学报(JSouthMedUniv )2010;30(11 压应力对组织工程软骨细胞增殖的影响 李殿威袁周 强袁郭 平袁宋 磊袁刘 涌渊第三军医大学西南医院骨 袁重庆400038冤 摘要院目的 探讨不同强度的压应力对组织工程软骨细胞增殖的影响遥 方法将兔软骨细胞接种到脱钙骨基质支架上袁 应用仿生多功能组织工程组织培养仪袁 加压组分别在压缩幅度为0~5%尧0~10%尧0~20%袁 频率0.1Hz周期性压力下培 养袁对照组不加压遥通过大体观察尧组织切片尧MTT法及流式细胞术分别观察组织形态尧细胞数量与分布尧检测细胞增殖 活力及细胞周期遥 结果0~10%组的组织工程软骨体积最大尧最厚袁形态佳袁组织切片见软骨细胞数目最多袁分布更为均 匀袁排列紧密袁基质分泌较多遥加压组的软骨细胞活力显著增强袁S期细胞百分比均明显高于对照组袁以0~10%组尤为明 显遥 结论组织工程软骨细胞增殖能力受周期性压力强度的调控袁其中频率0.1Hz尧强度0~10%的周期性压力能更好地 促进软骨细胞增殖遥 关键词院压应力曰软骨细胞曰细胞增殖曰组织工程 中图分类号院R318.01曰R322.71 文献标志码院A 文章编号院1673-4254渊2010冤11-2530-04 Proliferationoftissue-engineeredcartilagecellsundercompressivestress LIDian-wei,ZHOUQiang,GUOPing,SONGLei,LIUYong DepartmentofOrthopedics,SouthwestHospital,ThirdMilitaryMedicalUniversity,Chongqing400038,China Abstract: ObjectiveToinvestigatetheproliferationoftissue-engineeredcartilagecellsstimulatedbydifferentintensitiesof compressivestress. MethodsHumanembryoniccartilagecellswereseededintotypeIIcollagenspongescaffold.Thecellsof thepressuregroupswerestimulatedbydifferentcompressionrate(0-5%,0-10%, and0-20% )atacyclicalfrequencyof0.1 Hz.Thecellsofthecontrolgroupwereculturedwithoutpressure.Grossobservation,histologicalsection,MTTassayandflow cytometrywereusedtoobservethemorphology, cellnumberanddistributionanddetectthecellproliferationandcellcycle. Results Tissue-engineered cartilage cells in 0-10% group showed the largest size and greatest thickness with normal morphology. Tissuebiopsiesshowedthelargestnumberofcartilagecellswithmoreuniformdistribution, closealignment, morematrixsecretion. ThecartilagecellactivitywassignificantlyenhancedandthepercentageofSphasecellssignificantly increasedinthepressuregroupcomparedwiththoseinthecontrolgroup,andsuchchangeswereespeciallyobviousin0-10% groupinwhichtheSphasecellsincreasedby59.0%comparedwiththatinthecontrolgroup. ConclusionTheproli