SPS建立PTSD样大鼠旷场行为的改变及十味温胆汤的调节作用.pdfVIP

中国科技论文在线 SPS 建立 PTSD 样大鼠旷场行为的改变及十 味温胆汤的调节作用# 毛萌，宋月晗，李峰，刘燕，吴凤芝** 5 （北京中医药大学基础医学院，北京 100029） 摘要：目的观察单次延长应激(single-prolonged stress, SPS)建立PTSD 模型对实验大鼠模后 7 天后的旷场行为的变化以及中药复方十味温胆汤对其的调节作用。方法 采用旷场实验， 观察不同处理组大鼠的行为改变情况。结果 模型组大鼠在直立次数、穿格次数、运动总距 离、中央格停留时间方面均明显低于正常大鼠 (P0.05) , 西药阿普唑组大鼠，经统计在直立 10 次数、穿格次数、中央停留时间方面与模型组有明显差异（P0.05 ），而十味温胆汤组大鼠 各项指标均明显高于模型组，其中穿格次数、中央格停留时间与模型组具有非常显著的差异 （P0.01 ），且与正常组及西药组接近（P0.05 ）。结论本课题成功的复制了 PTSD 样大 鼠模型，所选取的中药复方十味温胆汤能显著改善PTSD 样大鼠的情绪行为变化，其效用与 西药阿普唑仑类似。 15 关键词：中医诊断学；创伤后应激障碍；单次延长刺激；十味温胆汤；旷场实验 中图分类号：Q-93-33 Effects of Shiweiwendantang on the behavior responses of PTSD rats established by SPS in the Open field 20 MAO Meng, SONG Yuehan, LI Feng, LIU Yan, WU Fengzhi (Basic Medical School, Beijing University, Beijing 100029) Abstract: In this paper,investigate the behavior changes of PTSD rats f or seven days and the influence of three Chinese prescription Shiweiwendantang. Methods The behavior change of rats treated by different method in the open field was observed. Results After the PTSD model was 25 establisded for 7days, rearing times ,times of spanning lattice ,standing times in the central area and total movement distance on the open field of rats in model group were significantly lower than that in normal group. These parameters in rats of Xanax group were all significantly higher than that in model group(P0.05) except the total movement distance （P0.05 ）.Meanwhile , these parameters of Shiweiwandantang group are all higher than