Solexa深度测序技术检测miRNA在性成熟猪睾丸中的表达情况.pdfVIP

中国科技论文在线 miRNA profiling in the sexually mature porcine testes determined by Solexa deep sequencing# Zhou Jiawei, Liu Gang, Guan Kaifeng, Ma Changping, Lei Bin, Tong Keya, Li Jialian, ** 5 Li Fenge (Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, WuHan 430070) Abstract: miRNAs are a large family of small regulatory elements that direct messenger RNA 10 degradation or disrupt mRNA translation. High-throughput Solexa sequencing approach was adopted to expand the repertoire of porcine miRNAs and to explore their roles on spermatogenesis. 237,590 total reads (792 unique reads) representing 65 known mature miRNAs in the miRBase release 14.0 were detected. 116 miRNAs including 12 known miRNAs in the miRBase release 15.0 and 104 novel miRNAs were identified from the un-annotated small RNAs. 466,757 targets for 104 novel miRNAs 15 were predicted by MIREAP. The highly expressed miRNAs in porcine testes (let-7c, let-7f, miR-148a, miR-140* and miR-199b) had a very high rate of SNP mutation, and the most popular SNP was T to G mutation at position 6. Three known miRNAs including miR-514, miR-507, miR-508-5p were