豆渣蛋白组分的特征性分析.pdfVIP

中国科技论文在线 豆渣蛋白组分的特征性分析 徐赏，华欲飞，陈业明** （江南大学食品学院，江苏无锡 214000 ） 5 摘要：本实验主要通过十二烷基硫酸钠—聚丙烯酰胺凝胶电泳 （SDS）的手段研究了 豆渣蛋白(OP)成分的特性。研究表明，与大豆分离蛋白 （SPI ）相比，豆渣蛋白含有 3 种特 征性蛋白条带，分子量分别约为27.21,16.00 和 23.38kDa 。基质辅助激光解析电离飞行时间 质谱(MALDI-TOF-MS) 的测定结果表明，分子量为27.21 和 23.38kDa 的蛋白质为碱性7s 球 蛋白 （Bg7s ），而分子量为23.38kDa 的蛋白质为油体蛋白 B 。豆渣蛋白的非还原、还原电 10 泳及对角线电泳结果显示，分子量为 50.10 和 34.27kDa 的豆渣蛋白含有分子间二硫键， MALDI-TOF-MS 鉴定其分别为11s 球蛋白和 Bg7s 。豆渣蛋白的三大组分为—残留的贮藏蛋 白 （7s 和 11s ），Bg7s 和油体蛋白 B ，其相对浓度分别约为57%,31%,12%。 关键词：豆渣蛋白；特征性；SDS；MALIDI-TOF-MS 中图分类号：TS214.2 15 Compositional Characterization of Okara Proteins XU Shang, HUA Yufei, CHEN Yeming (Food School, JiangNan University, JiangSu WuXi 214000) Abstract: This experiment mainly studies the compositional characterization of okara proteins 20 (OP)by SDS. The results show that, when compared with Soy Protein Isolates(SPI),okara protein contains three characteristic protein bands, whose molecule weight(MW) are respectively 27.21,16.00 and 23.38kDa. By MALIDI-TOF-MS, the 27.21 and 16.00kDa protein were identified as subunits of basic 7s globulin(Bg7s), while the 23.38kDa protein were identified as oleosin isoform B. Research results of reducing, non-reducing electrophoresis and diagonal 25 electrophoresis show that there are two proteins which are linked by subunits through disulfide bond, and they are identified as Bg7s and 11s globulin. The comparative concentration of remaining storage