冷冻干燥对婴儿双歧杆菌损伤作用的研究.pdfVIP

104 2009, Vol. 30, No. 01 食品科学 ※基础研究 刘丽凤，孟祥晨* (东北农业大学乳品科学教育部重点实验室，黑龙江哈尔滨 150030) 冷冻干燥是乳酸菌和益生菌干燥的主要方式，冷冻和干燥两个过程都会影响菌体细胞的存活性，本研究 主要目的是探讨冷冻和干燥过程对婴儿双歧杆菌的损伤作用。采用透射电镜观察菌体在冷冻干燥过程中的微结构， + + 通过溢出胞外的β- 半乳糖苷酶活性评价冷冻和干燥后细胞膜通透性的变化，通过测定冷冻和干燥后胞内Na 和K 浓 度的变化研究离子效应的影响。研究结果表明：婴儿双歧杆菌在冷冻干燥过程中的形态、细胞膜通透性和胞内离 子浓度变化程度随冻结时间的延长而加剧，冷冻干燥过程使菌体出现细胞质浓缩、质壁分离、菌体皱缩以及破损 现象，使细胞膜通透性增大，使胞内N a + 、K + 浓度显著下降，并伴随比例失调。 冷冻干燥；婴儿双歧杆菌；损伤 Damage of Bifidobacterium infantis Caused by Freeze-drying LIU Li-feng ，MENG Xiang-chen* (Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China) Abstract ：Freeze-drying is a major way to produce lactic acid bacteria and probiotic powders, and both freezing and drying can influence the cell survival. In order to evaluate the damage of Bifidobacterium infantis caused by freeze-drying, microstructure, + + β-galactosidase activity and concentrations of Na and K were determined in this experiment. The results showed that the changes + + of microstructure, β-galactosidase activity and concentrations of Na and K increase during freezing and drying processes, which include concentration of cytoplasm, separation between cytoplasm and cellular wall, shrinking and damage of cell, increase of cell + + membrane permeability, decrease and disparity between Na and K concentrations. Key words ：freeze-drying ；Bifidobacterium infantis ；damage 中图分类号：TS201.3 文献标识码：A