红姬凤梨试管苗生根影响因子及移植基质的探讨.pdfVIP

安徽农业科学,Journal of Anhui Agri .Sci .2008 ,36( 19) :8019 - 8020 ,8022 责任编辑 郑丹丹 责任校对 卢瑶 红姬凤梨试管苗生根影响因子及移植基质的探讨 惠惠 ( 安徽农业大学林学与园林学院, 安徽合肥230036) 摘要 [ 目的] 确定红姬凤梨试管苗生根 的最佳培养基及适宜的移栽基质 。[ 方 法] 以初代 培养的红姬 凤梨试管 苗为试验材料, 研 究不 同浓度IAA 、NAA 和IBA 对试管苗不定根诱导的影响及不 同基质配比对红姬凤梨试管苗移栽成 活率的影响。[ 结果] 在 生根培养基添加 不 同浓度IAA 、NAA 和IBA 的9 种处理 中, 以添加IAA 6 .0 mg/ L 处理的红姬凤梨生根数量最多, 平均根数达到23 .26 条, 平均根长达3 .41 c m; 在8 个不 同基质配比组合 中, 以泥70 % + 河沙30 % 组合的红姬凤梨移栽成 活率高, 幼苗生长较好, 表现为叶片宽阔、较厚、亮绿 色, 茎 平均增高1.58 c m。[ 结论] 红姬凤梨试管苗最佳生根培养基为 1/ 2 MS + I AA 6 .0 mg/ L + 蔗糖30 g/ L , 最适移栽基质 为泥炭70 % + 河沙 30 % 。 关键词 红姬凤梨; 生根; 移植基质 中图分类号 S682 .39 文献标识码 A 文章编号 0517 - 66 11(2008) 19 - 08019 - 02 Study on the Rooting Factors and Transpl anting Substrate of Crypt anthus aculis Plantlets HUI Hui ( College of Forestry and Landscape Gardening , Anhui Agricultural University , Hefei , Anhui 230036) Abstract [ Obj ective] The research ai med to confir mthe opti mum mediumfor rooting and the suitable transplanting substrate of Crypt anthus aculis plantlets . [ Method] Using the initially cultured plantlets of C . aculi s as tested materials , the effects of IAA ,NAA and I BA with different concn . on the adventitious root induction of C . aculi s plantlets and the effects of different substrate matching on transplanting survival rate of C . aculi s plantlets .[ Re- sult] Among 9 treat ments of rooting medium with IAA ,NAA and I BA at different concn . , the rooting number of C . aculis was the most i n the treatment with IAA at 6 .0 mg/ L , and the average root number was 23 .26 and the average length of root was 3 .41 c m. Among 8 combinations with different substrate matching , the proper substrate co mposition with 70 % peat + 30 % riversand was suitable for the transplanting survival and the young plants grow well , showing as that the leaves were thick and bright