苏丹Ⅰ与血清白蛋白的相互作用特征.pdfVIP

《化学通报》在线预览版 苏丹Ⅰ与血清白蛋白的相互作用特征 1,2 1 * 1 1 1 周能 梁逸曾 程前 龚雪斌 王永灿 1 中南大学化学化工学院 中草药现代化研究中心 长沙410083 2 玉林师范学院化学与生物系 玉林 537000 摘 要 利用荧光技术研究了在人体生理酸度条件下，苏丹Ⅰ与牛血清白蛋白相互作用，发现苏丹Ⅰ对牛血清白蛋白有较 强的荧光猝灭作用，用Stern-Volmer和Line weaver-Burk方程处理荧光猝灭数据，得到了反应的结合常数、结合热力学性质 和结合位点等参数。根据热力学常数确定了该药物与血清白蛋白之间的作用力类型，在此基础上依据福斯特Förster非辐射 能量转移机理探讨了苏丹Ⅰ与BSA相互结合时其供体-受体间的距离。 关键词 苏丹Ⅰ 牛血清白蛋白 荧光光谱法 Interaction Characteristic of Sudan Ⅰand Bovine Serum Albumin 1,2 1,* 1 1 1 Zhou Neng , Liang Yizeng , Cheng Qian ，Gong Xuebin , Wang Yongcan 1 Research Center of Modernization of Chinese Medicines, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083 2Department of Chemistry- Biology, YuLin Teachers College, Yulin 537000 Abstract The interaction between Sudan I and bovine serum albumin (BSA) was studied on the physiological conditions by using fluorescent technique. It is shown that Sudan Ⅰhas a powerful ability to quench the BSA’s fluorescence. The fluorescence quenching data were analyzed according to Stern-Volmer equation and Line weaver-Burk equation, and the binding constants, thermodynamic parameters and binding sites were obtained. According to the thermodynamic parameters the main sorts of binding force were ascertained ．The distance between BSA and Sudan Ⅰ was also given based on the Förster energy transfer theory ． Key words Sudan I, Bovine serum albumin, Fluorescence spectrum 苏丹Ⅰ（Sudan I，分子式：C H N O ）也称为苏丹红（一号），其化学名为 1-苯基偶氮-2-萘酚。国际 16 12 2 癌症研究机构（IARC ）将苏丹一号归为第三类可致癌物质，这类物质是缺乏足够的直接使人类致癌证据， 但是具有潜在致癌危险的物质。添加在食品中的