高血压疾病时血液系统L┐精氨酸载体转运动力学的改变.docVIP

高血压疾病时血液系统L┐精氨酸载体转运动力学的改变 作者：王军　徐洪涛　姚兴海　臧益民　唐朝枢 【关键词】 高血压 　　关键词: 高血压;红细胞;血小板;L-精氨酸 　　摘 要:目的 探讨原发性高血压（EH）时人体血液系统L-精氨酸（L-Arginine，L-Arg）载体转运功能的改变. 方法 EH患者12例及健康成年人16例，提取血小板（Pt）和红细胞（RBC），分别测定Pt和RBC的L-Arg转运的动力学特征. 结果 EH患者Pt和RBC对3 H-L-Arg转运呈高亲和性载体转运方式，在不同浓度点EH患者的3 H-L-Arg转运率均比对照组明显低下（Plt;0.01）.Eadie-Hofstee Plot分析，EH患者Pt L-Arg的最大转运速率（V max ）为对照组的79%（Plt;0.05），而Pt3 H-L-Arg转运的米氏常数值（Km ）无明显差异.RBC的L-Arg转运总能力Y+ 载体转运系统的转运能力明显低于正常组（Plt;0.01），而Y+ L载体转运系统的转运能力无明显改变.L-Arg总转运的最大速率与Y+ 载体转运系统的最大速率分别较对照组降低36.8%和42.5%（Plt;0.01），各组之间Km 无明显差异. 结论 EH患者Pt和RBC L-Arg载体转运系统存在明显异常. 　　Keywords:hypertension;erythrocyte;platelet;L-arginine 　　Abstract:AIM To investigate the changes of L-arginine 　　（L-Arg）transportation kinetic characteristics in erythrocytes（RBC）and platelets（pt）of patients with essential hyperten-sion（EH）.METHODS 12EH patients were studied with16normotensive people as control.The RBC and Pt were prepared and the transportation of L-Arg was measured by using the method described by Ghigo and Deliconstantins.RESULTS In EH patients，the ability of Pt L-Arg trans-portation was lower than control and Vmax decreased by21%（Plt;0.01）.In RBC，Saturable influx of L-Arg was mediated by teh classical cationic amino acid transport system Y+ and Y+ L system with high affinity.Under zero-trans condition，the ability of L-Arg transportation in EH patients was lower than control.The Vmax for total L-Arg transport and via sys-tem Y+ decreased36.8%and42.5%respectively compared with control（Plt;0.01）.But Vmax for L-Arg transport via system Y+ L was not affected by EH.There was no obvious difference between the groups.CONCLUSION There might be disturbance of L-Arg transportation in human erythrocytes and platelets of patients with EH. 　　0 引言 　　高血压病是人类死亡的重要原因之一，其发病机制仍未完全阐明.高血压时血液系统功能的改变尤其血小板（Pt）与红细胞（RBC）功能的变化及由此而引起的血液动力学指标的异常是其重要并发症.血细胞功能改变在血栓形成和动脉粥样硬化及高血压发病过程中的作用逐渐受到人们的重视.研究［1，2］ 表明Pt与RBC内均存在L-精氨酸（L-Arg）载体转运系统，通过合成的一氧化氮（NO）