IL-1β、IL-6对人冠脉平滑肌细胞表达基质金属蛋白酶-3的影响.docVIP

IL-1β、IL-6对人冠脉平滑肌细胞表达基质金属蛋白酶-3的影响 作者：蒋知新，夏爱祥，张清华，方效民，李运田，杜大勇，赖晓辉 【摘要】 目的 探讨炎症因子白介素-1β(interleukin-1β, IL-1β)、白介素-6(interleukin-6, IL-6)对人冠脉平滑肌细胞表达基质金属蛋白酶-3(matrix metalloproteinase-3, MMP-3)的影响。方法 ①采用20 μg/L的IL-1β、10 μg/L的IL-6各自刺激人冠脉平滑肌细胞，共同培养0、2、4、8、24、36 h后收集细胞；②采用不同质量浓度的IL-1β(0、5、20、40 μg/L)、IL-6(0、5、10、50 μg/L)刺激人冠脉平滑肌细胞，共同培养6 h后收集细胞。③应用实时荧光定量PCR方法检测细胞内MMP-3基因的表达量。结果 ①在同剂量IL-1β、IL-6刺激下，MMP-3的表达量在2 h时就开始发生上调，8 h达高峰，而后开始下降；②在不同剂量IL-1β、IL-6刺激下，MMP-3的表达量在实验剂量范围内随着剂量的加大呈上升趋势(IL-1β：r=0.907，P=0.000；IL-6：r=0.919，P=0.000)。各因子不同剂量组MMP-3的表达量均具有显著性差异(IL-1β：F＝24.047，P=0.000；IL-6：F＝14.081，P=0.001)。IL-1β 20 μg/L和40 μg/L组间MMP-3表达量没有显著性差异(P=0.154)，IL-6 5 μg/L和10 μg/L组间MMP-3表达量没有显著性差异(P=0.292)。结论 炎症因子IL-1β、IL-6能促进冠脉平滑肌细胞中斑块稳定相关标记物MMP-3的表达，这可能是炎症在急性冠脉综合征发生发展中的重要作用机制之一。 【关键词】 急性冠脉综合征；白介素-1β；白介素-6；基质金属蛋白酶-3；炎症；荧光定量聚合酶链反应 　　ABSTRACT: Objective To explore the effect of IL-1β and IL-6 on MMP-3 gene expression in human coronary artery smooth muscle cells. Methods We used IL-1β (20 μg/L) and IL-6 (10 μg/L) to stimulate human coronary artery smooth muscle cells, which were co-culture for 0, 2, 4, 8, 24, 36 h. IL-1β (0, 5, 20, 40 μg/L) and IL-6(0, 5, 10, 50 μg/L) were used to stimulate human coronary artery smooth muscle cells, which were co-cultured for 6 h. Then we detected the gene expression by fluorescent quantitation PCR. Results In the same concentration of IL-1β and IL-6, gene expression was up-regulated at 2 h, at 8 h the expression reached the peak, then began to descend. In different concentration of IL-1β and IL-6, gene expression was up-regulated with the dose of IL-1β and IL-6 (IL-1β: r=0.907, P=0.000; IL-6: r=0.919, P=0.000). There were significant differences in MMP-3 expression among different groups (IL-1β: F＝24.047, P=0.000; IL-6: F＝14.081, P=0.001). There were no significant differences in matrix metalloproteinase-3 between IL-1β 20 and 40 μg/L groups (P=0.154) and between IL-6 5 μg/L and 10 μg/L (P=0.292). Conclusion It suggests that IL-1β and