α连环素和β连环素在非小细胞肺癌中的表达及其临床意义.docVIP

α连环素和β连环素在非小细胞肺癌中的表达及其临床意义 【摘要】 目的 探讨α连环素(αcat)和β连环素(βcat)在非小细胞肺癌(NSCLC)中的表达及其与NSCLC的发生、发展及临床预后的关系。方法 应用免疫组化法，观察43例NSCLC患者及7例正常肺组织中的αcat和βcat的表达。结果 在43例NSCLC中，αcat和βcat的异常表达率分别为72.1%和65.1%；αcat、βcat异常表达与不同病理分级间和淋巴结有无转移均有显著性差异(Plt;0.05)；αcat和βcat异常表达者的生存时间明显少于正常表达者，βcat异常表达具有独立的预后意义(Plt;0.05，相对危险度RR=0.330)。结论 αcat和βcat表达与NSCLC的发生、发展及预后关系密切，可作为评估NSCLC进展及预后的指标。 【关键词】 肺肿瘤；α连环素；β连环素；免疫组织化学 ABSTRACT: Objective To investigate the expressions of αcatenin (αcat) and βcatenin (βcat) and their relation with development and prognosis of nonsmall cell lung cancer (NSCLC). Methods Expressions of αcat and βcat were immunohistochemically studied in 43 specimens of NSCLC and 7 cases of normal lung tissues. Results Of the 43 NSCLC cases, the abnormal rate of αcat and βcat expression was 72.1% and 65.1%, respectively. Differentiation and lymph node involvement had a significantly positive correlation with abnormal expression of αcat or βcat (Plt;0.05). The survival time was significantly shorter in patients with αcat or βcat abnormal expression than in those with normal αcat or βcat expression. βcat could serve as an independent prognosis factor (Plt;0.05). Conclusion The expressions of αcat and βcat are closely correlated with the occurrence, development and prognosis of NSCLC. αcat and βcat may be used as indicators for development and prognosis of NSCLC. KEY WORDS: lung neoplasm; αcatenin; βcatenin; immunohistochemistry 　 上皮细胞钙依赖黏附分子E钙黏素(Ecadherin)主要介导上皮细胞之间的连接和黏附，Ecadherin主要位于细胞膜上，与细胞膜内侧的连环素α、β结合成复合体，Ecadherincatenin(Ecadcat)复合体中任何组分结构和功能的异常均会影响细胞的黏附性[1]。近年来发现Ecadcat复合体功能异常与恶性肿瘤的侵袭转移、预后密切相关[2]。为此，我们应用免疫组化方法观察了αcat、βcat在非小细胞肺癌(NSCLC)中的表达，并探讨其表达与NSCLC发生、发展及预后的关系。 1 资料与方法 1.1 材料 选取西安交通大学医学院第一附属医院1994～1999年经根治性肺癌切除、术后病理证实的NSCLC石蜡包埋组织标本43例，其中男性35例，女性8例，男女之比为4.38∶1；年龄32～77岁，平均58.7岁；生存期最长的为72个月，且继续存活，最短于术后3个月死亡，死亡原因均系肺癌及相关疾病或并发症。所有病例术前均未行放疗和化疗，无其他疾病及并发症。病理类型：腺癌26例，鳞癌17例；分化程度：Ⅰ、Ⅱ级24例，Ⅲ级19例。临床分期(pTN