【化学工程与工艺专业】【毕业设计+开题报告+文献综述】磁性微球固定DNA的电化学生物传感器的研究.doc

（20 届） 毕业设计 磁性微球固定DNA的电化学生物传感器的研究 摘要: EDC/NHS活化，与探针DNA结合，在特定的条件下与待检测的DNA单链相结合，形成双链DNA，加入耐尔兰作为杂交指示剂，使其镶嵌到DNA螺旋结构的间隙里，采用常规示差脉冲伏安法（DPV)进行测定。检测线性范围为1.0×10-6mol/L ~1.0×10-10mol/L，检出限为4.2×10-11moI/L。实验结果表明本法响应迅速，特异性好，灵敏度高，检测简单方便。 关键词: Abstract: In this paper,presents a mark of hyperbranched polymers based on the conventional method of differential pulse detection。 First, hyperbranched polymers cured by chemical bonds at the surface of nano-magnetic beads,After EDC / NHS activation,Then with the corresponding target cells reaction,in certain conditions to be detected with the combination of single-stranded DNA,formation of double-stranded DNA,into NILE BLUE embedded into the DNA helix structure to the gap,used DPV to detection. The linear response of the sensor to hydrogen peroxide was in the concentration range of 1.0×10-6 mol/L ~1.0×10-10 mol/L, with a detection limit of 4.2×10-11moI/L.Experimental results showed that enlarged the electric sign and exhibited fast response, high sensitivity and good stability，detection of simple and convenient. Keywords: Nano-magnetic beads; DNA detection; NILE BLUE; Hyperbranched polymers目 录 摘要 I Abstract II 1 绪论 1 1.1 选题背景 2 1.2 相关研究成果 3 2 实验部分 5 2.1 实验内容 5 2.2.1 实验仪器 5 2.2.2 实验试剂 5 2.2方法分析......................................................................................................................................6 2.2.1 指示剂的配制 7 2.2.2 酰胺磁性微球的清洗 7 2.2.3 超支化聚合物的清洗 7 2.2.4 酰胺磁性微球和超支化聚合物的活化 ......................................................................7 2.2.5 实验步骤..........................................................................................................................8 2.2.6 电化学检测......................................................................................................................8 3 结果与分析 9 3.1 纳米电化学DNA传感器嵌合耐尔兰后的电化学行为 9 3.2 超支化聚合物的表征 10 3.3 水浴杂交时间优化 11 3.4 杂交温度的选择 11 3.5 线性关系.检测限和变异系数 12 3.5.1 线性关系 12 3.5.2 检出限 14 3.5.3 变异系数............