榄香烯和姜黄素诱导晶状体上皮细胞凋亡的体外研究.DOCVIP

临床医学论文-榄香烯和姜黄素诱导晶状体上皮细胞凋亡的体外研究 ???????????????????? 作者:黄秀榕, 祁明信, 康可人???? 【关键词】? 榄香烯;,姜黄素;,晶状体;,上皮细胞；,凋亡 　　摘要: 目的? 探讨榄香烯(Ele)和姜黄素(Cur)对体外培养的牛晶状体上皮细胞(LEC)凋亡的诱导效应及其机制。方法 将培养的牛LEC分别与160 μg/mL Ele和20 μg/mL Cur在CO2培养箱中共同孵育8,16,24,48,72 h,通过透射电子显微镜观察LEC超微结构;采用流式细胞术(FCM)测定LEC的DNA含量及线粒体跨膜电位(ΔΨm)。结果?? LEC分别与Ele和Cur共同孵育后,电子显微镜下可观察到细胞核染色质凝集、固缩、边集、核碎裂等,均呈现典型的细胞凋亡的形态学改变。FCM分析可见典型的凋亡亚G1峰,提示细胞核内DNA含量下降,并随药物作用时间的延长而加强。FCM检测发现细胞质出现线粒体ΔΨm下降,且在药物作用早期已发生变化。结论 Ele和Cur能显著诱导LEC凋亡;细胞核内DNA含量下降、细胞质内线粒体ΔΨm降低,分别是Ele和Cur诱导LEC凋亡的细胞核和细胞质途径。线粒体ΔΨm下降是Ele和Cur诱导LEC凋亡的早期事件。 　　关键词: 榄香烯; 姜黄素; 晶状体; 上皮细胞； 凋亡 　　ABSTRACT: Objective? To investigate the effect and the mechanism of elemene(Ele) and Curcumin(Cur) on apoptosis of lens epithelial cell(LEC) in vitro.Methods The bovine LEC cultured with 160 μg/mL Ele and 20 μg/mL Cur were observed by using transmission electron microscopy. The changes of DNA content and mitochondrial transmembrane potential(ΔΨm) in LEC were detected by flow cytometry. Results The typical morphological changes of LEC apoptosis in Ele and Cur group were observed under transmission electron microscope, such as chromatin condensation and aggregation at the nuclear periphery, and nuclear fragmentation as well. The DNA content of LEC in Ele and Cur group decreased timedependently.??????? The ΔΨm of LEC in Ele and Cur group decreased from early stage. Conclusion Ele and Cur can remarkably induce apoptosis of LEC in vitro. The decrease of DNA content in LEC nucleus and the collapse of mitochondrial transmembrane potential(ΔΨm) in cytoplasm are the nucleu and cytoplasm events of apoptosis mechanisms. The decreased of ΔΨm induced by Ele and Cur is the early event of LEC apoptosis. 　　KEY WORDS: elemene; curcumin; lens; epithelial cell; apoptosis 　　白内障囊外摘除联合人工晶状体植入术是目前白内障手术的主要方法,其近期疗效已十分肯定。但术后发生的后囊混浊,又称后发性白内障,导致远期视力下降是其主要的并发症之一。白内障术后残留的晶状体上皮细胞(lens epithelial cell,LEC)在晶状体囊膜上的增生、移行、产生胶原,是发生后囊混浊的主要原因。寻求高效低毒的防治后发障的药物具有重大意义[1]。有研究表明,从中药莪术中提取的挥发油榄香烯(elemene,Ele)可阻滞细胞从DNA合成期(S期)进入合成后期/分裂期(G2/M期),抑制