阿托伐他汀对鼠动脉粥样硬化基质金属蛋白酶和C-反应蛋白的影响.DOCVIP

临床医学论文-阿托伐他汀对鼠动脉粥样硬化基质金属蛋白酶和C-反应蛋白的影响 ??????????????? 作者：章义利 戴凌燕 周秀云 应斌宇 张怀勤???? 【摘要】? 　　目的 探讨阿托伐他汀对动脉粥样硬化鼠主动脉基质金属蛋白酶2、9（MMPs）及血中C-反应蛋白（CRP）的影响。方法 将雌性SD大鼠50只分为对照组(ｎ=10,正常饮食)、模型组(ｎ=20,维生素D3+高脂饲料+正常饮食)、阿托伐他汀组(ｎ=20,维生素D3+高脂饲料+阿托伐他汀)。4个月后处死，取主动脉起始部行光镜和电镜观察，其余部分用于免疫印迹法检测MMPs的表达水平。于实验前及实验结束时对实验鼠行空腹股动脉取血,分离血清,检测血清胆固醇(TC)、甘油三脂(TG)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)水平以及CRP的水平。 结果 实验前各组大鼠血脂学及CRP水平差异无显著性，实验结束时模型组TC、TG、LDL显著高于对照组（P0.01）, 阿托伐他汀组低于模型组(高于对照组)；MMP-2、MMP-9相对值模型组8618±549和9054±350，明显高于对照组的表达(对照组5314±163和6178±254)，而阿托伐他汀组3397±453和6805±368，低于模型组(P0.01)；CRP模型组（18.64±0.94)mg/L，显著高于对照组的（9.21±0.2）mg/L，而阿托伐他汀组(12.52±0.65)mg/L，显著低于模型组（P0.01）。对照组主动脉病理学改变比阿托伐他组显著，对照组改变不明显。 结论 阿托伐他汀能抑制基质金属蛋白酶和CRP的表达，在稳定粥样斑块过程中可能起重要作用。 【关键词】? 动脉粥样硬化 阿托伐他汀 基质金属蛋白酶 C-反应蛋白 　　【Abstract】? Objective? To investigate the effect of atorvastatin on expression of C-reactive protein (CRP) and matrix metalloproteinase-2,9 in experimental atherosclerosis of rats. Methods? Fifty female Sprague-Dawley rats were randomly divided into normal diet group (n=10,control group); vitamin D3 injection and high cholesterol diet group (n=40). After 8 weeks, vitamin D3 injection and high cholesterol diet rats were randomly switched to received atorvastatin (5mg.kg-1.day-1) (n=20,atorvastatin group) or normal diet (n=20, model group).Another eight weeks later, all the rats were killed and some of their aortas were observed by light and electron microscope, the left aortas were removed for western blot analysis to detect MMP-2,-9 . At the begin and end of experiment, serum was collected for serum lipid and CRP determining. Results?There were no significant changes in serum lipids and CRP at the beginning of experiment between the groups ,But after two months, Cholesterol, low density lipoprotein(LDL), triglyceride(TG) of atorvastatin group were significantly lower than those of model group(P0.01) but higher than control group(P0.01). The pathologic changes of atorvastatin group were less serious than those of model group, there was n