菜素对慢性间歇低氧幼鼠认知损害的保护作用.DOCVIP

临床医学论文-菜素对慢性间歇低氧幼鼠认知损害的保护作用 【摘要】? 目的:研究芹菜素对慢性间歇低氧幼鼠认知损害的保护作用。方法:八臂迷宫训练成功的3～4周龄雄性SD幼鼠40只，随机分空气对照组(C组)、间歇低氧组(H组)、间歇低氧二甲基亚砜组(R组)、间歇低氧芹菜素+二甲基亚砜组(Q组)。间歇低氧2周后八臂迷宫测试全部幼鼠学习记忆能力。以透射电镜观察海马CA1区神经元的超微结构变化。结果:与R组比，Q组参考记忆错误(RME)和总记忆错误(TE)显著降低(P＜0.05，P＜0.01)。与C组比，H组RME、工作记忆错误(WME)和TE显著增高(P＜0.01，P＜0.05，P＜0.01)。电镜下H组和R组海马CA1区神经细胞的形态、胞浆内的细胞器与正常细胞比较有明显变化，Q组和C组无明显变化。结论:芹菜素对于慢性间歇低氧幼鼠海马神经元具有保护作用，从而减轻慢性间歇低氧对幼鼠空间学习记忆能力的损伤。 【关键词】? 慢性间歇低氧 芹菜素 记忆 海马 超微结构 ??? Abstract:? Objective: To explore the protective effect of apigenin in immature rats treated with chronic intermittent hypoxia. Methods: Forty 3～4 week-old SD rats which have been trained to complete the 8-Arm (4-arm baited) radial maze were randomly divided into air control(C) group, intermittent hypoxia(H) group, DMSO control(R) group and apigenin + DMSO(Q) group. After 2 weeks intermittent hypoxia, these immature rats were tested by 8-Arm (4-arm baited) radial maze to measure spatial memory and the ultrastructure of hippocampal CAl neurons was studied under electron microscope. Results: Compared with R group, Q group had significantly less in reference memory error and total memory error (P＜0.05, P＜0.01).Compared with C group, H group had significantly reference，working and total memory error, respectively（P＜0.01, P＜0.05, P＜0.01).The nuclear membrane of hippocampal CAl neurons in H group and R group were distorted compared with normal cells; Q group and C group had no changes. Conclusion: Apigenin protects hippocampal neuron in immature rats, and improves spatial learning and memory damaged by chronic intermittent hypoxia. 牋?Key words:?chronic intermittent hypoxia; apigenin; memory; hippocampus; ultrastructure ??? 芹菜素是天然存在的一种黄酮类化合物，有研究显示芹菜素对氧自由基、NO自由基等均有一定的清除作用，可抑制导致细胞死亡的氧化作用,从而体现了神经保护的作用[1]。但其应用于防治阻塞性睡眠呼吸暂停综合征(OSAHS)儿童认知损害方面的报道较少。本试验模拟OSAHS的病理生理基础，建立慢性间歇低氧的幼鼠模型，并用芹菜素进行干预，采用八臂迷宫测试其学习记忆能力，以透射电镜观察海马的神经细胞超微结构，观察芹菜素干预慢性间歇低氧幼鼠认知损害的效果并探讨可能的作用机制。 ??? 1? 材料和方法 ??? 1.1? 动物与药品? 取体重为80～100 g健康雄性SPF级SD幼鼠(3～4周龄)50只，由温州医学院实验动物中心提供，合格证号：SYXK(浙)200