miR 374过表达增强人乳腺癌细胞对多西他赛的耐药性.pdfVIP

9·90 · 韦伊芳，等 miR-374过表达增强人乳腺癌细胞对多西他赛的耐药性 miR-374过表达增强人乳腺癌细胞对多西他赛的耐药性 韦伊芳，张 媛，马勇政，药立波，张 健 ｍｉＲ -374 ｅｎｈａｎｃｅｓ ｄｏｃｅｔａｘｅｌ ｒｅｓｉｓｔａｎｃｅ ｏｆ ｂｒｅａｓｔ ｃａｎｃｅｒ ｃｅｌｌ ｌｉｎｅ WeiYifang，ZhangYuan，MaYongzheng，YaoLibo，ZhangJian DepartmentofBiochemistryandMolecularBiology，theFourthMilitaryMedicalUniversity，ShaanxiXian710032，China. 【Abstract】 Objective：ToinvestigatetheeffectsofmiR-374overexPressionondocetaxeldrugresistanceofbreast cancercelllineMDA-MB-231.Methods：TherelativeexPressionsofmiR-374inMDA-MB-231-Che yand MDA-MB-231-miR-374celllineswereidentifiedbyqPCR.ThecellswerePlantedinto96-wellPlatesand treatedwithdifferentconcentrationofdocetaxel，seventy-twohourslate ，cellviabilitywasdetectedusingMTTas- say.MDA-MB-231-Che yandMDA-MB-231-miR-374cellsweretreatedwithdocetaxelatafinalconcen- trationof3.2nmol/L，andcolony-formingassaywasusedtodetectthecellProliferationactivity.ThecellaPoPtosis rateandcellcycleweredetectedbyflowcytometry.Results：TherelativeexPressionofmiR-374inMDA-MB- 231-miR-374wassignificantlyhigherthanthatinMDA-MB-231-Che ycells.ComParedwithcontrolcells， theProliferationandcolony-formingabilitiesofMDA-MB-231-miR-374cellsimProvessignificantly，thePer- centagesofcellaPoPtosisdecreased，thecellsatG /G Phasedecreased，andthoseatSandG /MPhasesincreased， 0 1 2 andtheaPoPtosisrateofcellsdecreasedsignificantly.Conclusion：TheoverexPressionofmiR-374maysignificantly enhancebreastcancerMDA-MB-231cellstodocetaxelresistance. 【Keywords】miR-374；MDA-MB-231；drugresistance；docetaxel ModernOncology2014，22（05）：0990-0993 【摘要】 目的：观察miR-374过表达对人乳腺癌MDA-MB-231细胞docetaxel（多西他赛）耐药性的影响。 方法：qPCR检测miR-374过表达细胞MDA-MB-231-miR-374 和对照细胞MDA-MB-231-Che y miR-374 的相对含量；采用不同浓度的多西他赛处理细胞72 小时