威灵仙注射液对骨关节炎模型动物软骨组织形态和胶原表达的影响.DOCVIP

临床医学论文-威灵仙注射液对骨关节炎模型动物软骨组织形态和胶原表达的影响 ???????????????? 作者： 陈飞雁，王旭，黄加张，夏军，顾湘杰 【关键词】? 骨关节炎 ??? ?摘? 要：［目的］探讨威灵仙注射液对骨关节炎模型动物关节软骨的组织学、胶原表型和超微结构的作用。［方法］建立骨关节炎动物模型，动物随机分为3组，S组、D组和K组，S组和D组分别注射威灵仙注射液和生理盐水，K组空白。分批处死动物取关节软骨标本，常规石蜡切片，HE／SOFC双染色，Mankin评分比较各组关节软骨损伤程度。免疫组化法（IHC）检测各组关节软骨基质Ⅰ、Ⅱ型胶原的表达情况。透射电镜（TEM）观察各组关节软骨超微结构变化。［结果］S组在各时段的评分均优于D组和K组；S组在各时点Ⅰ型胶原阳染面积低于D组和K组，Ⅱ型胶原阳染面积高于D组和K组；S组软骨在各时间点损伤程度都低于D组和K组，K组和D组损伤程度基本一致。［结论］威灵仙注射液可维持和促进软骨合成蛋白多糖与Ⅱ型胶原，对关节软骨具有保护作用。 ?? 关键词：骨关节炎；? 威灵仙注射液；? 组织学；? Ⅱ型胶原；? 超微结构 ?? Abstract：［Objective］To study the effect of sixpetal clematis root injection（WLX）on histology,collagen phenotype and ultramicrostructurc in cartilage of osteoarthritis animal model［Method］OA model animals were randomly divided into 3 groups:group S,group D and group KAnimals in group S received WLX and animals in group D received sterilized normal saline intramuscularly,group K was control and received nothingAfter the animals were killed,cartilage specimen were obtained and sections were made and stained with HE/SOFG for Mankin score;the expression level of collagen Ⅰ and Ⅱ in matrix was tested by immunohistochemistry;change of ultramicrostructure of cartilage was observed with transmission electron microscope［Result］Mankin score in group S was better than that in group D and K at each test period;result of IHC shows that positive stain area of collagen Ⅰ was lower in group S than that in group D and K,and positive stain area of collagen Ⅱ was higher than that in group D and K at each test period;cartilage impairment degree in group S was lower than in group D and K,and impairment manifestation in group D and K was similar on the whole［Conclusion］sixpetal clematis root injection may protect articular cartilage by maintaining and promoting the chondrocyte synthesizing proteoglycan and type Ⅱ collagen ?? Key words：Osteoarthritis；? WLX；? Histology；? Type Ⅱ collagen；? Ultramicrostructure ?? 骨关节炎（OA）是一种多因素引起的以关节软骨退行性变为基本病理改变的疾病。延缓关节软骨退变和促进透明软骨再生是防治OA的关键。目前尚缺乏治疗OA的特异性方法。中药威灵仙是治疗风湿痹痛的传统药物，威