胸髓横断对大鼠膀胱神经支配通路的影响―假狂犬病毒跨突触示踪研究.DOCVIP

临床医学论文-胸髓横断对大鼠膀胱神经支配通路的影响―假狂犬病毒跨突触示踪研究 【摘要】 目的 利用假狂犬病毒跨突触示踪技术，研究胸髓横断伤所致的急、慢性脊髓损伤（SCI）对膀胱脊髓神经支配通路的影响。方法 76只SD大鼠随机分为4组:non-SCI、SCI? a 、SCI? b 和SCI? c 组。SCI? a 、SCI? b 和SCI? c 组大鼠完全横断脊髓，在饲养不同时间后，膀胱壁内注射假狂犬病毒（PRV）;non-SCI组大鼠不损伤脊髓，仅于膀胱壁组织内注射PRV。各组注射PRV后于既定时间点经心脏灌注固定取材。采用链霉卵白素―过氧化物酶（SP）免疫组织化学方法显示背根神经节（DRG）和脊髓切片中PRV阳性神经元。计算机图像分析软件统计各组PRV阳性神经元数目和面积，单因素方差分析比较各组统计值差异。结果 non-SCI、SCI? a 、SCI? b 和SCI? c 组大鼠膀胱重量分别为（144±9）mg、（142±8）mg、（486±51）mg和（656±69）mg。non-SCI和SCI大鼠PRV示踪的时限性和分布相似。SCI大鼠病毒标记的DRG细胞面积显著增加（P0.001），non-SCI、SCI? a 、SCI? b 和SCI? c 组分别为（593±40）μm 2 、（588±39）μm 2 、（815±53）μm 2 和（902±57）μm 2 。慢性SCI大鼠在注射PRV后第3天，L 6 和S 1 节段后角标记细胞数显著增加;注射后第4天，L6 和S 1 节段标记的运动神经元数也显著增加。结论 急性和慢性脊髓损伤不影响大鼠膀胱的PRV示踪特性。慢性SCI大鼠膀胱排尿反射通路出现显著重组。 　　关键词 脊髓损伤 膀胱 排尿反射通路 假狂犬病毒 跨突触示踪? ???? 　　Effect of thoracic cord transection on the urinary bladder spinal neural pathway in rats:a transsynaptic tracing study by PRV?????? 　　【Abstract】 Objective To determinate the effect of acute and chronic spinal cord injury（SCl）resulted from thoracic cord transection on the urinary bladder spinal neural pathway with a transsynaptic tracing study using Pseuˉdorabies Virus（PRV）.Methods 76adult Sprague-Dawley rats were randomly divided into4groups，non-SCI，SCI? a ，SCI? b and SCI? c respectively.The non-SCI rats underwent no surgical procedure except pseudorabies vires（PRV）tracer injection into the bladder tissue while the rats of other groups were spinalized and given PRV injection at differˉent time after SCI.Transcardiac perfusion fixation was done in appropriate survival periods after PRV injection.Then sections of dorsal root ganglion（DRG）and spinal cord were processed for visualization of virus by the Streptavidin-Peroxidase（SP）immuno-histochemical procedure.All sections were measured with the Olympus Cue-2image analˉysis system.One way analysis of Variance test was used for comparison of different measurements between normal and spinalized rats.Results The bladder weights in non-SCI，SCI? a ，SCI? b and SCI? c rats were