选择性COX2抑制剂NS398对前列腺癌PC3细胞增殖与凋亡的影响.DOCVIP

临床医学论文-选择性COX2抑制剂NS398对前列腺癌PC3细胞增殖与凋亡的影响 【摘要】? 目的 观察环氧化酶2(COX2)抑制剂NS398对前列腺癌细胞PC3增殖和凋亡的影响。方法 采用四甲基偶氮唑蓝法(MTT法)检测不同浓度和不同时间NS398对PC3细胞增殖的影响；RTPCR法检测不同浓度NS398作用PC3细胞24h后COX2 mRNA的表达；酶联免疫测定法(ELISA)检测不同浓度NS398作用PC3细胞24h后PGE2释放水平；流式细胞仪检测不同浓度NS398作用PC3细胞24h后细胞凋亡情况。结果 NS398可以抑制PC3细胞的增殖，呈时间和剂量依赖性；RTPCR和ELISA法检测结果显示，随着NS398浓度增高，PC3细胞COX2 mRNA表达和PGE2释放水平呈下调趋势；细胞凋亡检测结果显示100、200μmol/L NS398对PC3细胞具有诱导凋亡的作用。结论 NS398可能通过COX2依赖性途径抑制前列腺癌PC3细胞增殖，促进肿瘤细胞凋亡。 【关键词】? 前列腺癌；环氧化酶抑制剂；增殖；凋亡 ??? ABSTRACT: Objective? To investigate the effect of cyclooxygenase2 inhibitor NS398 on the proliferation and apoptosis of prostate cancer cell line PC3 in vitro. Methods? The proliferative inhibition of PC3 cells was observed by methyl thiazol tetrazolium(MTT)rapid photocolorimetric assay; the expression of COX2 mRNA in cultured PC3 cells was examined by RTPCR; the release levels of PGE2 was measured by enzymelinked immunosorbent assay(ELISA); PC3 cells cultured in different concentrations of NS398 medium for 24h was marked with Annexin VFITC and PI for the cell apoptosis assay by flow cytometry. Results? After the PC3 cells were treated with different concentrations of NS398, the inhibitory rate on proliferation increased with the increasing of concentration and days. The expression of COX2 mRNA and the release levels of PGE2 were decreased compared with those of the control group. PC3 cells cultured in 100 and 200μmol/L NS398 medium for 24h had significantly higher incidence of apoptosis. Conclusion? NS398 can inhibit proliferation of human prostate cancer cell line PC3 and induce its apoptosis in vitro, which may contributed to the COX2 dependent pathway. ??? KEY WORDS: prostate cancer; cyclooxygenase inhibitor; proliferation; apoptosis??? 　? 环氧化酶2(COX2)是近年来国内外研究的热点，其在包括前列腺癌等多种恶性肿瘤中呈阳性表达[1]，因而选择性阻断或抑制COX2的活性，可能有利于前列腺癌的预防和治疗。我们利用选择性COX2抑制剂氮2，环己氧4，硝基苯甲基磺胺(NS398)干预前列腺癌PC3细胞，观察其对前列腺癌细胞增殖和凋亡的影响，并初步探讨其可能存在的机制。 ??? 1? 材料与方法 ??? 1.1? 材料? 人前列腺癌细胞株PC3细胞(中国科学院上海细胞生物研究所)；N