重组人生长激素对裸鼠人胃癌移植瘤细胞周期的影响.DOCVIP

临床医学论文-重组人生长激素对裸鼠人胃癌移植瘤细胞周期的影响 ???????? 作者：梁道明　张毅　张荧荧　袁勇　周东　陈嘉勇? 【摘要】? 目的：研究重组人生长激素（recombinant human growth hormone, rhGH）对裸鼠人胃癌移植瘤细胞周期的影响。方法：将24只实验裸鼠随机分为对照组、顺铂（DDP）组、rhGH组和DDP+rhGH组，每组6只，将体外培养的人胃癌细胞MKN45接种于裸鼠右臀部皮下，裸鼠胃癌移植瘤模型成功建立后开始连续给药6 d，给药结束后处死裸鼠。取出移植瘤，用流式细胞仪检测细胞周期，并分析细胞周期、细胞增殖指数（PI）及DNA抑制率。结果：给药结束后DDP+rhGH组和DDP组S期细胞明显减少，而rhGH组和对照组比较增多（P0.05）；G0-G1期、G2-M期的细胞增殖指数（PI）和DNA抑制率各组间比较差异均无统计学意义（P0.05）。rhGH组细胞凋亡率增加而对照组、DDP组和DDP+rhGH组减少（P0.05）。结论：未发现rhGH对裸鼠人胃癌移植瘤细胞有明显促进分裂增殖作用。 【关键词】? 重组人生长激素·裸鼠·胃肿瘤·细胞周期 ??? Effects of recombinant human growth hormone on cell cycle of human gastric carcinoma xenograft in nude mice ??? 【ABSTRACT】Objective:To study the effects of recombinant human growth hormone （rhGH） on cell cycle of human gastric carcinoma xenograft in nude mice. Methods:The nude mice of this experiment were divided into control group, rhGH group, cisplatin （DDP） group and DDP+ rhGH group. Human gastric carcinoma cells MKN45 were inoculated in right buttock of nude mice and carcinoma xenograft model was established, the drugs were continuously given to nude mice in the following 6 days. All animals were sacrificed after the experiment was finished. The tumor inhibition rate was counted by measuring tumor volume. Cell cycle of human gastric cancer MKN45, cell proliferation index （PI） and DNA? in? hibitional? rate? were? analyzed? by? flow? cytometry? （FCM）. Results: The? cells? in? S phase? of? DDP+ rhGH? group? and? DDP? group decreased obviously compared with those of rhGH group and control group（P0.05）.There? was? no? statistical? difference? among? cells? in? G0-G1? phase,? G2-M? phase, PI? and DNA inhibition? rate. The? apoptosis? cells? in? rhGH? group? increased? remarkably （P0.05）. ??? 　　近年来，生长激素（growth hormone, GH）作为一种与合成代谢有关的物质已受到广泛的关注，临床应用GH可纠正手术及创伤后的负氮平衡状态，能显著改善患者的营养状况,促进胃肠道管壁细胞的增殖和修复。已有研究证实GH在氮平衡时水平升高，而在伤口感染及术后下降［1］。但术后应用GH在促进机体恢复的同时是否会促进肿瘤细胞的增殖，目前仍缺乏实验依据。本研究通过在裸鼠体内建立移植瘤模型来研究重组人生长激素（recombinant human growth hormone, rhGH）对人胃癌细胞是否具有增殖作用，为临床胃癌患者术后是否能应用rhGH提供实验依