转录因子T-bet，GATA-3对食管癌患者免疫状态的影响.DOCVIP

临床医学论文-转录因子T-bet，GATA-3对食管癌患者免疫状态的影响 【摘要】? 目的： 研究食管癌患者转录因子T-bet，GATA-3的基因表达及其对患者免疫状态的影响。方法： 根据其CT分期将40例食管癌患者分为两组：一组为第Ⅰ，Ⅱ期患者，另一组为第Ⅲ，Ⅳ期患者；荧光定量PCR技术检测40例食管癌患者及20例健康对照者外周血单核细胞T-bet，GATA-3 mRNA的表达水平；ELISA法检测血浆中辅助性T细胞1型(Th1)细胞因子IFN-γ及辅助性T细胞2型(Th2)细胞因子IL-4。结果： 食管癌组较对照组T-bet mRNA表达明显降低，GATA-3 mRNA表达明显升高，IFN-γ表达明显降低，IL-4表达明显升高，不同期别食管癌组间差异不显著。T-bet，GATA-3分别与IFN-γ，IL-4呈正相关。结论： 食管癌患者Th1型细胞因子表达降低，Th2型细胞因子表达升高，即Th1/Th2平衡被打破，发生了向Th2方向的漂移，免疫功能受损；这种改变受到转录因子T-bet，GATA-3的调控，T-bet表达的减少在这种调控中可能起着关键的作用。 【关键词】? 食管癌； 转录因子； 细胞因子； T-bet； GATA-3 　　［Abstract］? Objective: To evaluate the expression of transcription factors? T-bet/GATA-3 mRNA in patients with esophageal carcinoma and their influence on those patients′ immunity.Methods： According to CT image,patients were divided into two groups, stage I plus II, and, stage Ⅲ plus Ⅳ; The gene expression of T-bet/GATA-3 in patients with esophageal carcinoma and control group was detected by reverse transcription-polymerase chain reaction RT-PCR.The expression of IFN-γ and IL-4 for Th1/Th2 cytokine was measured by ELISA.? Results： T-bet mRNA expression of esophageal carcinoma was significantly lower than that in control, the expression of GATA-3 mRNA in esophageal carcinoma was significantly enhanced. The plasma level of Th1 cytokine IFN-γ in esophageal carcinoma was significantly lower than that in control.The level of Th2 cytokine IL-4 in esophageal carcinoma was significantly higher than that in control. However，the difference between two groups was not significant.? Conclusion： In patients with esophageal carcinoma, we found there were lower production of IFN-γ for Th1 and over-production of IL-4 for Th2,which meant there was imbalance between Th1 and Th2.The change that the imbalance differentiation of Th cells was regulated by T-bet and GATA-3,and the less T-bet expression may be a more important factor. 　　［Key words］?? esophageal carcinoma； transcription factors； cytokines； T-bet； GATA-3 　　食管癌是许多国家和地区常见的恶性肿瘤，而我国更是世界上食管癌死亡率最高的国家，近20年来，食管癌的治疗