木瓜粉对体外缺氧损伤大脑神经细胞及缺血性脑梗塞患者保护作用研究.pdf

功能均有所改善、夕 通过体内、体外两部分实验结果显示木瓜粉具有明显的抗氧化功能，能提 高缺氧损伤神经细胞的NSE,ACHE活性，MTT和DNA含量，改善缺血性脑 梗塞患者血脂状态，对体外鼠胚大脑神经细胞缺氧损伤恢复及缺血性脑梗塞患 者功能恢复都具有促进作用。 关键词 木瓜粉、 神经细胞、 脑梗塞、一 缺血 缺氧 抗氧化 体内 体外 Abstract AsstrokeisbecomingoneofthemajorcausesofdisabilityanddeathinChina, thereisgreatinterestinthebasicmechanismsbywhichischemia/reperfusion(I/R) causesdamage.Itsconfirmedbyalotofresearchthatitisrelatedwithrfeeradicals Papayapowder(Bio-Normalizer,BN)isanaturalsupplement,whichisawhite, sweet,multipleplantenzymegranulesproductedrfom fermentationofCarica papaya,itsreportedthatitisarfeeradicalscavenger,andexhibitantioxidant activity.Inthisstudy,weinvestigatedtheprotectiveeffectofBNonratembryonic cerebralneurocytesinvitroandtheantioxidativeeffectofBNoniscbemiccerebral infarctionpatientsinvivo. Theratembryoniccerebralneurocytesweredividedintofourgroups(Igroup anoxicdamageOmgBN/ml,11groupanoxicdamageO.1mgBN/ml,IIIgroup anoxicdamage0.5mgBN/mlandNgroupnon-anoxicdamageOmgBN/ml).The growth,developmentandmorphologyofneurocyteswereobservedbythelightand electronicmicroscope.Theactivityofneuronspecificenolase(NSE)and acetylcholineesterase(ACHE),theDNA contentofneurocytesandMTTof neurocytesweredetected.TheresultsshowedthattheactivityofNSE,ACHE,DNA contentandMTTofneurocytesinanoxicdamageO.1mgBN/mlgroupwere significantlyhigherthanthatinanoxicdamageOmgBN/mlinvitro(P0.05).The morphologicalobservationshowedthattherecoveryofanoxicdamageinanoxic damageO.1mgBN/mlandanoxicdamage0.5mgBN/mlgroupswassignificantly betterthanthatinanoxicdamageOmgBN/mlgroupinvitro.Inaconclusion,the papayapowder(BN)canpromotetherecoveryofanoxicdamageofratembryonic cerebralneurocytesinvitro. Acase-controlclinicaltrialwascarriedon60ischemicstrokepatientsaged 50-75,oraladministrationofBNorplacebo(VitC)wasstartedimmediatelyafter admissionandlastedfortwomonths.Theactivityoferythrocytesuperoxide dismutase(SOD),