缬沙坦对糖尿病大鼠肾皮质ICAM1、VCAM1表达作用研究.pdfVIP

较变化较轻。电镜观察发现B组在实验第4周出现基底膜不 规则增厚，系膜区增宽，足突细胞增生，足突融合，毛细血 管受压甚至闭塞，第6周上述变化更加显著，A组与同时期B 组比较病变明显减轻、延缓。RT-PCR半定量结果分析显示， B组 ICAM-1及VCAM-ImRNA表达较A,C组显著增高 (P0.01)，，A组表达较C组为高 (P0.01)、但仍较B组为 低 (P0.01)。I,- 结论:血管紧张素11(AngII)受体拮抗剂缄沙坦能够减 少糖尿病大鼠的尿白蛋白排泄，下调肾皮质 ICAM-1及 VCAM-ImRNA表达，减轻肾脏肥大及延缓肾小球硬化，具有 保护肾脏的作用。 关键词: 撷沙坦 糖尿病肾病 细胞间粘附分子一1 血管间粘附分子一1 EffectofAngiotensinIIReceptorAntagonist onExpressionofICAM-1,VCAM-1inRenal CortexofExperimentalDiabeticRats Abstract Objective: It isforinvestigatingthemechanismof Valsartaninprotectingthekidneyofstreptozotocin-induced diabeticrats. Methods:Sprague-dawleyratswererandomlydividedto diabetic model treated with Valsartan (10mg.kg- /d)group(A),diabeticmodelgroup(B)and normalcontrol group(C).Plasmaglucose,kidneymass/bodymassratio,MAP, seurmcreatinine,urinarycreatinine,24urinaryalbuminexcretion rateweremeasuredinthe4th,6thweekrespectively.ICAM-land VCAM-1mRNA expression inrenalcortinalweremeasured withRT-PCRin6thweek.Andthekidneysamplewereobserved withlightandelectronmicroscope.Meanglomeurlartransverse sectionalareaandmeanglomeurlarvolumewerecalculatedby analysissystemoftheimage. Results:Ineithertwostages,kidneymass/bodymassratio, Ccr,24urinaryalbuminexcretionrateinbothdiabeticgroup旧) werehigherthanthatofnormalgroup(C)(P0.01),buttheywere decreasedsignificantlyinAgroupthanthatinBgroup.Forthe urinaryalbuminintwostages,therewasnodifferencebetweenA and C(P0.05).In4and6,theMGPAandMGVin A weresignificantlysmallerthanthatofBgroup(P0.01).The electronmicroscopicfindingsindicatedthatbothgroupsof diabeticratsshowedsignificantmesangialespansionandfeet- synapsefusion,glomerularbasementmembranethickeningand disappearanceoftheendothelumcellwindowsandsoonin differentstages,buttheabnormalchangeswerealleviatedinA group. Conclusion: Valsartancouldreducetheexpressionof 1CAM-1and VCAM一ImRNA,itcouldreducethelevelof urinaryalbuminandcou