腰椎间盘突出症患者突出髓核中抗原抗体复合物的测定及临床意义.pdfVIP

腰推间盘突出症患者突出翻核中抗原抗体复合物的测定与临床意义 摘 要 目的:探讨抗原抗体复合物在突出髓核中的分布及临床意义。 方法:应用免疫酶组织化学染色的方法，对31例腰椎间盘突出 症患 的突出髓核中抗原抗体复合物进行检测，与20例正常椎间 盘作对照并结合临床资料分析其意义。 结果:在突出髓核的标本中可在髓核细胞膜周围观察到显示阳 性的棕色沉淀物存在，而在正常椎间盘对照组中却未见到阳性显 示。突出型及脱垂游离型髓核中68.42%显示为强阳性，31.58%显示 为阳性，膨隆型髓核中58.33%显示为阳性，41.67%显示为阴性。两 组之间P0.05有统计差异 结论:在突出髓核组 普遍存在抗原抗体复合物，而健康椎 间盘中却不含有此免疫复合物。抗原抗体复合物阳性与强阳性表达 与髓核暴露于机体免疫系统的程度相关，临床疼痛症状的程度与自 身免疫反应的程度相关。髓核突出后引起的自身免疫反应是导致神 经根的慢性炎症的重要原因。 关键词:免疫组织化V髓核突LJ腰稚 搜推间盘突出症患者突出位核中抗原抗体复合物的测定与临床意义 摘典 Abstract AIM:Toassessthedistributionofantigen-antibodycomplexes anditsclinicalsignificanceintheherniatednucleuspulposus. METHOD:Thepresenceanddistributionofantigen-antibody complexesweredetectedin31casesofherniatednucleuspulposus妙 themethodofimmunohistochemistry.The resultswerecompared withthoseofcontrolsubjectsandanalyzedinconnectionwithclinical finding. RESULTS:A brown deposit ,indicating antigen-antibody complexescouldbeobservedinthepericellularcapsuleinherniated disctissuebutnotincontroldiscs.There wascleartendencyfora morepronouncedstainingofthepericellularcapsuleinnoncontained herniatedtissuesthanincontainedherniatedtissues.68.42%arestrong positive,31.58%positiveofnoncontainedherniatedtissues.Onthe contrast,58.33%containednucleusarepositive,41.67%negative.There isstatisticaldifferenceamongthem(P0.05). Conclusion:Antigenantibodycomplexesseemtobecommonly presentinherniateddisctissue,butnotinhealthydiscs.Theexpression degreeoftheAntigen-antibo勿complexesiscorrelationwiththe exposingdegreeoftheherniatednucleuspulposusintheimmune system.Experimentalevidenceexistthatthepainofpatientswillbe moreseverduringtheimmunologicreactionoftheantigen-antibody complexheaviercausingbytheherniatednucleuspulposus.Itindicates indicates theimmunologicreactionmightbeofimportantinthe pathophysiologiceventsleadingtonerveinjuryin