高温联合辐射对胚胎神经发育及脑热应激蛋白的影响.DOCVIP

临床医学论文-高温联合辐射对胚胎神经发育及脑热应激蛋白的影响 【关键词】? 高温；辐射；孕鼠胚胎；神经发育；脑热应激蛋白质 　　【摘要】 目的? 探讨高温联合辐射致胚胎神经发育与早期胚胎脑蛋白质和HSPs表达的关系。 方法? 将130只孕鼠按随机抽签分为13组，每组10只。在鼠妊娠第8d，第9d分别接受60Co及γ-射线全身照射各1次,照射剂量1.0Gy；第10d各置温箱中,分别使其肛温保持在37±0.5℃，41±0.5℃，42±0.5℃,并持续3，4，5min。移出温箱2h后，每组每时段随机取5只脱颈法处死，采用Western dot blot 和lowry方法定量分析胚胎脑HSP70和蛋白质含量与胚胎神经系统畸形。 结果? 高温联合辐射对胚胎神经毒性明显增加，并与高温强度及作用时间呈正相关；高温联合辐射作用于胚胎早期对胚胎脑蛋白质降低有复合作用（P＜0.01），并可诱导脑HSP70合成。 结论? 高温与辐射联合致神经发育与胚胎脑蛋白质合成及热应激蛋白的诱导有一定的相关性。 　　【关键词】 高温；辐射；孕鼠胚胎；神经发育；脑热应激蛋白质 　　Effects of hyperthermia combined with radiation on embryonic nervous development and heat shock protein of brain 　　【Abstract】 Objective? To explore the relation between the embryonic nervous development induced by hyperthermia combined with radiation and early embryonic brain proteins or HSPs expression. Methods? 130 pregnant mice were randomly divided into 13 groups( each n= 13).On the 8th? and? 9th? day of pregnance, the mice received one time of general irradiation of 60Co and γray respectively, the dosage of irradiation was 1.0 Gy;on the 10th? day ,the mice were put in? hotboxes? keeping their rectal temperature at 37±0.5℃, 41±0.5℃ and 42±℃0.5℃ persisting for 3,4 and 5 mins, respectively. After two hours of the mice leaveing warming boxes, 5 mice randomly selected from each group per timesection were executed by cervical vertebra luxation. The HSP70 of embryonic brain, protein content and deformations of embryonic nervous systems were quantitatively analyzed with the Western dot blot and Lowry method. Results?The toxicity of hyperthermia combined with irradiation to embryos markedly increased and was positively related to hyperthermia intensity and duration; hyperthermia with irradiation had compound action on reducement of embryonic brain proteins in early embryo (P0.01) and induced the synthesis of brain HSP70. Conclusion There was certain relativity of nervous development induced by hyperthermia combined with irradiation and synthesis of embryonic brain proteins or induction of HSP70.???