上皮性卵巢癌组织中乙酰肝素酶的表达及其与预后的关系.pdfVIP

现代肿瘤医学 2012年2月 第20卷第2期 MODERNONCOLOGY，Feb．2012，VOI．20，NO．02 ·357· 上皮性卵巢癌组织中乙酰肝素酶的表达及其与预后的关系 王福玲 ，娄艳辉 ，崔竹梅 ，李 翠 ，王 蕾 Theexpressionofheparanaseinepithelialovariancancerand itscorrelationwiththe prognosis WANG Fuling。LOU Yanhui。CUIZhumei，LICui，WANG Lei ， DepartmentofGynecology，theAffiliatedHospitalof QingdaoUniversityMedicalCollege，ShandongQingdao266003，Chi— na；DepartmentofGynecology，舭 PeoplesHospitalofMuping，ShandongYantai264100，China． A【bstract】 Objective：Toinvestigatetheexpressionofheparanase(Hpa)inepithelialovariancanceranditscorre— lationwithclinicalpathologicalfeatureandprognosisofthepatients．Methods：ExpressionofHpainovariancane— ers，benignovariantumorandnormalovarian tissuesweredetectedbyRT —PeR assayandimmunohistochemical staining．Results：PositiveexpressionrateofHpaintumortissueswas73．33％ ，significantlyhigherthanthatinbe— nigntumorsandnormaltissues(P0．05)．Hpaprotein—positivemainlylocatedincellkytoplasm，stainingintensi— tymainlywas(++)一(+++)，significantlystrongerthanthatincontrolgroup．TheexpressionofHpaandwas higherincanceroustissueswithlymphaticmetastasisthanthatwithoutlymphaticmetastasis(P0．01)．Thepositive rateofHpainstageIII—IVwashigherthanthatofpatientsinstageI—II(P0．O1)．Log—rankanalyzisindicated theaccumulatedsurvivalrateofthepatientswithHpaexpressionwaslowerthanthepatientswithoutHpaexpression (P0．05)．Conclusion：TheoverexpressionofHpamayplayanimportantroleinthedevelopmentandprorgession ofovariantumors．TheexpressionofHpacanbeusedasanusefulmarkerofthebiologicalbehaviorofovariancancer andanindepentprognosticfactorforovariancancer． 【Keymords】heparanase；epithelialov~Ylaikearlcerprognosis Modem Oncology2012，20(O2)：0357—0360 摘【要】 目的：研究乙酰肝素酶(Hpa)在上皮性卵巢癌组织中的表达，分析其与卵巢癌临床病理特征及预后 的关系。方法：采用RT—PCR和免疫组化方法检测乙酰肝素酶 mRNA及蛋