基础研究腺病毒介导的PML基因对银屑病样小鼠模型的作用.pdfVIP

·432 · doi 10.3969/j.issn. 1673-4254.2013.03.26 J South Med Univ, 2013, 33(3): 432-435 基础研究 腺病毒介导的腺病毒介导的PMLPML 基因对银屑病样小鼠模型的作用基因对银屑病样小鼠模型的作用 1 1 1 2 1 1 1 王琼玉 ，张爱军 ，马慧群 ，王世捷 ，马云云 ，邹兴伟 ，李蕊联 1 2 西安交通大学第二附属医院皮肤科，环境与疾病基因教育部重点实验室//环境与地方病研究室，陕西 西安710004 摘要：目的研究腺病毒介导的PML 基因对银屑病样小鼠模型的作用。方法 采用小鼠阴道上皮及鼠尾鳞片表皮实验模型，观 察PML 基因重组腺病毒乳膏外用对鼠尾鳞片及阴道上皮细胞分裂的影响。结果 PML 基因重组腺病毒乳膏具有显著抑制阴 道上皮细胞有丝分裂、促进表皮颗粒层生成的作用。结论 PML 基因对银屑病样小鼠模型的作用可能与促进表皮细胞正常分 化和抑制表皮细胞增殖有关，PML 基因局部外用有望用于银屑病等角化异常性疾病的治疗。 关键词：银屑病；PML 基因；动物模型 Effect of topical application of a recombinant adenovirus carrying promyelocytic leukemia gene in a psoriasis-like mouse model 1 1 1 2 1 1 1 WANG Qiongyu , ZHANG Aijun , MA Huiqun , WANG Shijie , MA Yunyun , ZOU Xingwei , LI Ruilian 1 Department of Dermatology, Second Affiliated Hospital , Key Laboratory of Environment and Genes Related Diseases, Institute of Environment and Endemic Diseases of State Ministry of Education2, Xian Jiaotong University College of Medicine, Xian 710004, China Abstract: Objective To investigate the effects of topical treatment with adenovirus-mediated promyelocytic leukemia gene (PML) gene in a psoriasis-like mouse model. Methods The effect of adenovirus-mediated PML gene on the granular layer of mouse tail scale epidermis and epithelial mitosis were observed on longitudinal histological sections prepared from the tail skin and vaginal epithelium of the mice. Results Adenovirus-mediated PML gene significantly inhibited mitosis of mouse vaginal epithelial cells and promoted the formation of granular layer in mouse tail scale epidermis. Conclusion The therapeutic effect of P