枯草芽孢杆菌群3－甘露聚糖酶基因克隆及同源性分析Cloning.pdfVIP

基因组学与应用生物学，2009 年，第28 卷，第5 期，第845-850 页 Genomics and Applied Biology, 2009, Vol.28, No.5, 845-850 研究报告 Research Report 枯草芽孢杆菌群 -甘露聚糖酶基因克隆及同源性分析 β 刘勇 毛爱军 李辉 程池* 中国食品发酵工业研究院, 中国工业微生物菌种保藏管理中心, 北京, 100027 * 通讯作者, cheng100027@163.com 摘 要 本文对33 株枯草芽孢杆菌群菌株进行 -甘露聚糖酶活性筛选，其中的32 株具有 -甘露聚糖酶 β β 活性，只有1 株无 -甘露聚糖酶活性。通过基因克隆测序的方法获得33 株枯草芽孢杆菌群菌株 -甘露聚 β β 糖酶基因编码区全序列，对酶基因进行同源性分析并构建系统发育树；在 -甘露聚糖酶基因系统发育树 β 中，33 株枯草芽孢杆菌群菌株聚为3 个分支，分别是枯草芽孢杆菌分支、地衣芽孢杆菌分支和解淀粉芽孢杆 菌分支；枯草芽孢杆菌、地衣芽孢杆菌和解淀粉芽孢杆菌 -甘露聚糖酶基因种内同源性大于91% ，而种间 β 同源性为60%~69%。 关键词 枯草芽孢杆菌群, β-甘露聚糖酶, 基因克隆, 同源性分析 Cloning and Homology Analysis of Beta-mannanase Gene from the Collec- tion of Bacillus subtilis Group Liu Yong Mao Aijun Li Hui Cheng Chi * China National Research Institute of Food Fermentation Industries, China Center of Industrial Culture Collection (CICC), Beijing, 100027 * Corresponding author, cheng100027@163.com DOI: 10.3969/gab.028.000845 Abstract The beta-mannanase activity of thirty-three strains from the collection of Bacillus subtilis group were determinated in this study, the result revealed that thirty-two tested strains exhibited beta-mannanase activity as well as only one strains beta-mannanase activity wasnt detected. The complete encoding sequences of beta-mannanase gene from thirty-three B. subtilis group strains were obtained by using gene cloning and sequencing, and the be- ta-mannanase gene homology was analyzed and a phylogenetic tree was generated. In the phylogenetic tree of the beta-mannanase gene, thirty-three B. subtilis group strains were clustered into three distinct clades, the B. subtilis , the . clade and the .