中国地方鸡种MHC B-LBⅡ新等位基因的遗传多态性研究.pdfVIP

Journal of Genetics and Genomics (Formerly Acta Genetica Sinica) February 2007, 34(2): 109-118 Research Article Characterization of Genetic Polymorphism of Novel MHC B-LB Ⅱ Alleles in Chinese Indigenous Chickens 1.2 1 3 2 4 1 1,① Rifu Xu , Kui Li , Guohong Chen , Hui Xu , Bayangzong Qiang , Changchun Li , Bang Liu 1. College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 2. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China; 3. Department of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; 4. Department of Animal Husbandry and Veterinary Medicine, College of Agriculture and Anim al Husbandry, Tibet University, Linzhi 860000, China Abstract : Genetic polymorphism of the major histocompatibility complex (MHC) B-LB Ⅱ gene was studied by amplification of exon 2 using PCR, followed by cloning and DNA sequencing in eight indigenous Chinese chicken populations. To reveal the ge- netic variation of the B-LB Ⅱ gene, 37 types of patterns detected by PCR-SSCP were investigated first, which would be used to screen novel B-LB Ⅱ sequences within the breeds. The types of PCR-SSCP patterns and final sequencing allowed for the identifica- tion of 31 novel MHC B-LB Ⅱ alleles from 30 unrelated individuals of Chinese chickens that were sampled. These are the first designators for the alleles of chicken MHC B-LB Ⅱ gene based on the rule of assignment for novel mammalian alleles. Sequence alignment of the 31 B-LB Ⅱ alleles revealed a total of 68 variable sites in the fragment of exon 2, of which 51 parsimony informa- ti