粘蛋白MUC1 568A_G SNP与辽宁地区人群胃癌遗传易感性的关系.pdfVIP

HEREDITAS (Beijing) 2008 9 , 30(9): 1163―1168 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2008.01163 MUC1 568A/G SNP 徐倩, 孙丽萍, 宫月华, 徐莹, 董楠楠, 袁媛 , 110001 摘要：为了探讨粘蛋白(MUC1)基因 568 位点 A/G 单核苷酸多态性与胃癌遗传易感性的关系, 采用序列特异性 引物-聚合酶链反应(Sequence specific primers PCR, PCR-SSPs)检测来自辽宁地区人群 138 例胃癌患者及与其配 比的131 例对照个体MUC1 568 位点A/G 多态性, 以ELISA 法检测血清H. pylori IgG 抗体。结果显示:(1)对照 人群MUC1 基因568 位点AA 、AG 、GG 3 种基因型分布频率分别为73.3% 、22.1 ％、4.6%; (2) 胃癌组MUC1 AA 基因型携带频率显著高于正常对照组(P=0.03), 携带MUC1 AA 基因型个体胃癌的发病风险增高到 1.92 倍; (3) 以MUC1 AG+GG 基因型并血清幽门螺杆菌(H. pylori)IgG 抗体阴性的个体为对照, AG+GG 基因型并H. pylori IgG 抗体阳性个体、AA 基因型并H. pylori IgG 抗体阴性个体、AA 基因型并H. pylori IgG 抗体阳性个体胃癌患 病风险增高, 但3 组各组间差异均无统计学意义(P0.05) 。说明MUC1 基因568 位点A/G 多态与胃癌的遗传易 感性相关; MUC1 A/G 基因多态性和H. pylori 感染在胃癌发生发展过程未见交互作用。 ：基因多态性; 粘蛋白MUC1 ; 胃癌; 幽门螺杆菌 The relationship between the polymorphism of MUC1 and suscepti- bility to gastric cancer in Liaoning region XU Qian, SUN Li-Ping, GONG Yue-Hua, XU Ying, DONG Nan-Nan, YUAN Yuan Cancer Control Laboratory of Cancer Institute and General Surgery Institute, the First Affiliated Hospital of China Medical University, Shenyang 110001, China Abstract: Analyzed the relationship between 568 site A/G polymorphism in mucin 1(MUC1) gene in a population from Liaoning Province and susceptibility to gastric cancer. Sequence specific primers-polymerase chain reaction(PCR-SSPs) were performed to analyze the genotype of the A/G polymorphism in its 568 site of exon 2 for 138 gastric cancer cases and 131 normal ones tested, and ELISA was performed to test IgG antibody of H. pylori . We found that the distribution fre- quency of AA, AG, GG three genotypes were 73.3%, 22.