风湿康治疗大鼠骨关节炎实验的研究.docVIP

风湿康治疗大鼠骨关节炎的实验研究 戚晶敏 吴启富 （南方医科大学中医药学院， 广州 510515） 【摘要】目的 探讨风湿康治疗大鼠膝骨关节炎的作用机制。方法 4%木瓜蛋白酶关节腔注射建立大鼠OA模型，1周后48只大鼠平均分成6组：正常组、模型组、双醋瑞英组，1.35 g/kg，2.7 g/kg，5.4 g/kg的风湿康治疗组，正常组与模型组给与等体积的生理盐水。4周后处死动物，检测血清中IL- 1β和TGF-β1的含量，切取关节，行大体和组织学观察，采用免疫组织化学方法检测基质金属蛋白酶-13(MMP-13)在关节软骨中的表达。结果 风湿康治疗组均能降低血清中IL -1β的水平（P＜0.001），风湿康中剂量和高剂量组能显著升高血清中TGF-β1的水平(P＜0.001)。风湿康低剂量组对关节软骨的保护作用并不明显（P＞0.05），风湿康中剂量和高剂量组均可显著抑制MMP-13在膝关节软骨中的表达（P＜0.05），风湿康高剂量组与双醋瑞英组具有相似的疗效（P＞0.05）。 结论 风湿康可能是通过降低血清中IL-1β，升高TGF –β1的水平，减少MMP -13在关节软骨中的表达，从而对骨关节炎发挥治疗作用。 【关键词】风湿康；骨关节炎；IL-1β；TGF –β1；MMP -13 Effect of feng shi kang on experimental osteoarthritis in rats QI Jing-ming WU Qi-fu ( School of Traditional Chinese medicine Southern Medical University , Guangzhou 510515) 【Abstract】Objective To investigate the mechanism of feng shi kang(FSK) for experimental knee osteoarthritis in rats. Methods Forty-eight rats were divided into six groups with eight in each as following：normal group，model group，Diacerein group and 1.35，2.7，5.4g/kg FSK group．Animal models of osteoarthritis were established by intra—articular injection of 4% papain in the knee of the rats in the 1atter five groups. The animals began to receive the corresponding drugs once a day in four weeks. The normal control and model groups received same amount of saline instead. Four weeks later，all animals were sacrificed for analysis. Macroscopic and histological examinations were performed on the articul arcartilage. Immunohistochemica1 and morphometric analysis were adopted to record the in situ expression and distribution of matrix metalloproteinases-13(MMP-13) in the cartilage．At the same time, the contents of interleukin-1β(IL- 1β)and transforming growth factorβ1(TGF-β1) in the serum were detected by ELISA. Results FSK groups could reduce the levels of interleukin-1β(IL- 1β)(P＜0.001 ), FSK at moderate and high concentrations could significantly increase the levels of transforming growth factorβ1( TGF-β