丁基苯酞对缺血脑组织中及低糖低氧刺激后培养的神经细胞中胆碱乙酰化酶活性的影响.pdfVIP

·药 　理· 丁基苯酞对缺血脑组织中及低糖低氧刺激后培养的神经细胞中胆碱乙 酰化酶活性的影响 ( ) 种兆忠 　冯亦璞 北京 100050 中国医学科学院中国协和医科大学药物研究所 ( ) 摘要 　目的 :观察丁基苯酞 dlNBP , dNBP 和 lNBP 对脑缺血后皮层和纹状体中以及低糖低氧和 NMDA 刺激后培养的胎 鼠皮层神经元中胆碱乙酰化酶活性变化的影响。方法 :大脑中动脉堵塞起始处造成大鼠局灶性脑缺血 ;胎鼠皮层神经元以低 糖低氧培养基培养造成低糖低氧模型 ;胆碱乙酰化酶活性以分光度法进行测定。结果 :脑缺血后缺血区皮层和纹状体中胆碱 乙酰化酶活性分别下降了61. 3 %和 58. 4 % 。dlNBP , dNBP 和 lNBP ( 10 和 20mg ·kg - 1 ,ip) 于脑缺血后 5 和 60min 给药 2 次 皆可显著提高脑组织中胆碱乙酰化酶活性。dlNBP , dNBP 和 lNBP (0. 1～10μ - 1 ) mol ·L 对低糖低氧及 NMDA 刺激后神经 细胞中胆碱乙酰化酶活性也有明显的提高作用。结论 : dlNMP 改善局灶性脑缺血后动物的学习记忆功能可能与其对胆碱能 神经功能的改善有关。 关键词 　丁基苯酞 ;脑缺血 ;神经元 ;低糖低氧 ;胆碱乙酰化酶 Effect of dl3nbutyl phthalide on the activity of the choline acetyltransferase in ischemic brain and cultured neurons subjected to hypoglycemia/ hypoxia Chong Zhaozhong (Chong ZZ) ,Feng Yipu ( Feng YP) ( Institute of M ateria Medica , Chinese Academy of Medical Sci ) ences , Peking U nion Medical College , Beijin g 100050 ABSTRACT　OBJECTIVE : To investigate the effects of dl3nbutylphthalide ( dlNBP) , dNBP and lNBP on the activity of choline acetyltransferase (ChA T) in ischemic brain after focal cerebral ischemia and in neurons subjected to hypoglgcemia/ hypoxia. METHODS :Focal cerebral ischemia was induced by blockade of the origin of middle cerebral artery (MCAO) and perinatal cortical rat neurons was cultured with hypohlycemia/ hypoxic medium to induce hypoglycemia/ hypoxia injur y. The activity of ChA T was deter mined spectrophotometrically. RESULTS :The results indicated that the activity of ChA T was decreased by 61. 3 % and 58. 4 % re spectively in cerebral cortex and striatum after six hours of MCAO. dlNBP , dNBP and lNBP ( 10