烟草内生芽孢杆菌降低烟叶亚硝胺类物质含量研究.pdfVIP

3. 烟草内生芽孢杆菌降低烟叶亚硝胺类物质含量的研究 (云南省烟草科学研究所，玉溪市，653100 ) 雷丽萍 摘要：从白肋烟TN90品种的主脉组织中分离到一株内生菌株WT。根据形态和生理生化特征进行鉴定。该菌株在 ： TSA培养基上菌落形态为白色，环状突起。对利福平和萘啶酮酸敏感。革兰氏阳性（G+ ）、催化酶活性，产生芽孢， 可运动，杆状，具一定的碱性磷酸酶活性和一定的脲酶活性，鉴定为芽孢杆菌(Bacillus sp.)。在温室移栽时将 该内生细菌接种于白肋烟 TN90, 并在收获后喷施细菌悬液于烟叶表面，进行亚硝胺类物质的检测，结果表明： 接种WT可减少白肋烟TN90的亚硝胺组分，TSNA总量比对照减少，叶片组织减少 21.7%～44.6%，主脉组织减少 16.7%～80%。整个根系接种可有效降低叶片和主脉组织内TSNA总量（P≤0.05），分别降低了38.0%和80%。叶面 喷施可使叶片组织内 TSNA 总量降低 44.6%，也明显影响叶片和主脉组织中 NNN 含量，差异显著（P≤0.05）。对 NAT和 NAB含量的影响不显著。 关键词：TSNA 烟草晾制 烟草内生细菌 Reduction of tobacco specific nitrosamine in air-cured tobacco leaves by a bacterial endophyte(Bacillus sp.) （Institute of Tobacco Science Research, Yuxi 653100）LEI Liping A putative tobacco bacterial endophyte, WT was isolated from Burley tobacco variety TN90, and it was characterized by morphological, physiological, and biochemical methods. The WT isolate formed circular, white, convex, raised colonies on tryptic soy agar medium that were extremely sensitive to rifampicin and nalidixic acid. It was Gram positive, catalase positive, spore-forming, motile rod, with limited alkaline phosphatase activity and limited nitrate-reducing ability. Identification of WT by substrate use patterns (BIOLOG™) was unsuccessful but the most likely genus in which to place the isolate is Bacillus sp.. TN90 was grown in a greenhouse and at the transplant stage inoculated with isolate WT, which was unable to reduce NO3- to NO2-. The grown tobacco was air cured and an additional treatment in which WT was sprayed on leaf tissue was employed. The total nitrosamine content and the content of individual nitrosamines was, on average, lower in inocula