水稻细卷叶突变体nrl2(t)的遗传分析应用和基因定位.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2011, 37(7): 1159− 1166 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2011.01159 水稻细卷叶突变体nrl2(t) 的遗传分析和基因定位 王德仲 桑贤春 游小庆 王 增 王秋实 赵芳明 凌英华 李云峰 何光华* / , 400715 : 10 EMS , nrl2(t) , , , , , SSR 3 SSR s3RM1 s3RM3 , 114 kb : (Oryza sativa L.); ; ; ; Genetic Analysis and Gene Mapping of a Novel Narrow and Rolled Leaf Mutant nrl2(t) in Rice (Oryza sativa L.) WANG De-Zhong, SANG Xian-Chun, YOU Xiao-Qing, WANG Zeng, WANG Qiu-Shi, ZHAO Fang-Ming, * LING Ying-Hua, LI Yun-Feng, and HE Guang-Hua Rice Research Institute, Southwest University / Chongqing Key Laboratory of Transgenic Plant and Safety Control, Chongqing 4007 15, China Abstract: Study of the genes which regulate leaf development is significant in rice functional genomics research and molecular breeding. In the present study, a novel rolled and narrow leaf mutant was obtained from the rice (Oryza sativa L.) restorer line Jinhui 10 treated by ethyl methyl sulphonate (EMS), named nrl2(t). The mutant exhibited narrow/semi-rolled and elongate leaves, earlier flowering, thinner stalk, higher pigment content and higher plant height compared with the control. The indole-3-acetic acid (IAA) content relatively reduced in the flag leaf compared to that of wild type, but increased in the young panicle at the heading stage. Genetic analysis indicated that the mutant was controlled by a single recessive gene, located on the chromosome 3 between SSR markers s3RM1 and s3RM3, delimiting to a 114 k