补肾活血法组方中药防治肾间质纤维化的实验研究_6248588百替生物.pdfVIP

补肾活血法组方中药防治肾间质纤维化的实验研究 Experimental study regarding to protectiVe and therape Utic effects 0f Nourishingkidney and ActiVing blood on renal interstitial fibrosis 张大宁 张勉之 ZHANG Da-ning ZHANG Mian-zhi 天津市中医药研究院 （中国 300120 ） Tianjin Academy of Traditional Chinese Medicine President (China 300120) 中图分类号：R322.6+2 文献标识码：A 文章编号：1818 －0086 （2009 ）03 摘 要 ：目的 探讨依据补肾活血法组方对进展性肾间质纤维化的防治作用。方法 制作大鼠肾间质 纤维化模型，将大鼠分为假手术组，高、低剂量治疗组和模型组。观察肾间质病变的程度及角蛋白、波形 蛋白和 α-平滑肌肌动蛋白在肾间质的表达情况。结果 治疗组肾间质损害程度明显轻于模型组(P0.01) ； 波形蛋白、α-平滑肌肌动蛋白阳性细胞表达明显少于模型组，角蛋白的表达明显多于模型组(P0.01)。结 论 补肾活血法中药使肌成纤维细胞的表达减少，上皮细胞的表达增强，说明抑制并逆转了肾小管上皮细 胞肌成纤维细胞转分化，阻止了肾间质纤维化的进程。 关键词 ：补肾活血法 ；肾间质纤维化 Abstract:Objective ：To investigate the protective and therapeutic effects of Nourishing kidney and Activing blood on prog~ssivefibrosis in renalinterstitial ．Methods The rat models of renal interstitial fibrosis，divided into pseudoperation group，therapeutic group andmodel group ．The pathological changes and expression of α-SMA，Vim and stiti in renal interstitial fibrosis ．Results The lesion of renal inter-therapeutic group were significantly better than those of thecontrol group(P0.01).The number of α-SMA and Vim positive expression in therapeutic group were lessthan those in model group,thenumber of CK was on the contrary (P0.01).Conclusion Apply theformula of Nourishing kidney and Activing blood can reduce the Myofibroblast expression and increase the epithelial cells expression ，inhibited and transformed TEMT ，blockage renal interstitial fibrosis ． Key Words:Nourishing kidney and Activing; blood Renal interstitial ;fibrosis Treatment 肾间质纤维化(renal interstitial fibrosis ，RIF)是各种肾脏疾病发展的最终结果；近年来的研究显示肾间 质病变与慢性肾衰竭关系较肾小球更为密切[1-2] 。补肾活血法(Nourishin kidney and Activing‘blood)是张大宁 氏于1978 年首先出的一种