温敏性壳聚糖_甘油磷酸二钠盐水凝胶的初步研究_黄彬.pdfVIP

10 () 2008 22 1!Journal ofXianning University(M edicalSciences) ∀ / 黄 彬, 陈建钢 (武汉大学中南医院, 湖北武汉 430071) :探讨温敏性壳聚糖/甘油磷酸二钠盐水凝胶应用于 骨再生的可行性 制备壳聚糖/-甘油磷 酸二钠盐水凝胶将体外培养的小鼠 骨细胞与水凝胶共培养, 采用荧光倒置显微镜观察细胞在三维材料上生长分布 的状态选取共培养 12周的细胞/水凝胶复合物,采用RTPCR半定量的方法, 检测型胶原和聚合素两种细胞外基质 在mRNA转录水平上的差异 制备的水凝胶具备生理性的 pH 值, 在常温下为液态,体温状态下为胶冻状荧光倒 置显微镜显示共培养2周时 骨细胞呈球状生长细胞/水凝胶复合物共培养2周后,型胶原和聚合素的合成增加 壳聚糖/甘油磷酸二钠盐水凝胶具有温度敏感性, 是一种生物相容性的温固化凝胶 : 壳聚糖; 甘油磷酸二钠盐; 温度敏感材料 :R318. 08 :A :2008 Study onNovelThermallySensitiveChitosan/glycerophosphateHydrogel HUANG Bin, CH N Jiangang ( Zhong nan H osp ital, Wuhan Un iv ers ity, Wuhan H ubei 430071, Ch ina ) ABSTRACT:ObjectiveTo investigate the feasibility of novel therm ally sensitive chitosan/- glycero phosphate (Ch/GP) hydrogel for chondrocytes regeneration. ethodsCh/GP hydrogelswere prepared. Fluo rescent inverted m icroscopy ( FIM ) was used to show the cellular proliferation and threedmi ensional distribu tion in the hydrogels. The expression ofwere collagen type and aggrecan detected with RTPCR. Results These formulations possess a physiologicalpH and can be held liquid below room temperature for encapsulating living cells; they form monolithic gels at body temperature. FIM shows that at 2weeks, the cells are generally round. In addition, we found that the normalized ratio of collagen type and aggrecan significantly increased at 2weeks, as compared to that at 1week(P 0. 05). ConclusionThis study reports for the first tmi e the use of Ch/GP aqueous solutions asgelling system s, suggesting the discovery of a prototype for a new fam ily of ther mosetting gels highly compatiblew ith biological compounds. KEYWORDS:Chitosan; glycerophosphate; Thermally responsivem aterial [ 5- 8] , , , , ,