Allelic diversification at the wx locus in landraces of Asian rice文献.pdfVIP

Theor Appl Genet (2008) 116:979–989 DOI 10.1007/s00122-008-0729-z ORIGINAL PAPER Allelic diversiWcation at the wx locus in landraces of Asian rice I. Mikami · N. Uwatoko · Y. Ikeda · J. Yamaguchi · H. Y. Hirano · Y. Suzuki · Y. Sano Received: 30 August 2007 / Accepted: 9 February 2008 / Published online: 28 February 2008 © Springer-Verlag 2008 Abstract To examine continuous variation of amylose from Taiwan) joined the japonica lineage; however, com- levels in Asian rice (Oryza sativa) landraces, the Wve puta- parisons using linked genes for two Taiwanese accessions a in b op revealed that the wx gene was the product of gene Xow tive alleles (Wx , Wx , Wx , Wx , and wx) at the wx locus were investigated in near-isogenic lines (NILs). Apparent from japonica to indica. Therefore, the japonica lineage amylose levels ranged from 0.5 to 29.9% in the NILs, frequently included Wxin b a op , Wx and wx, while Wx and Wx showing a positive relation with the levels of Wx gene prod- were found in the other lineages, strongly suggesting that uct, granule-bound starch synthase (GBSS) as well as the allelic diversiWcation occurred after divergence of the two enzymatic activity per milligram starch granule. Only subspecies. The present results were discussed in relation to opaque (Wxop) accessions had an enzymatic activity per the maintenance of agronomically valuable genes in vari- GBSS that was reduced to half the level of the others. ous landraces. Nucleotide sequences in the Wx gene were com