黄芪多糖对慢性阻塞性肺病大鼠肺组织HO_1表达的影响.pdfVIP

2010 12 36 24 J iang su M ed J, Decembe 2010, V ol 36, No . 24 2943 H O1 韩扣兰 李荣良 常唐喜 黄 诚 戴小丽 李卫勇 ! ( A PS) ( CO PD) ( H O) 1 60 5 , 12 : A CO PD , B1B2B3 A PS 102040 mg/ kg ; C ( L PS) CO PD RTP CR Weste n blot A PS HO1 mR NA 11530 d, A H O1 mR NA C ( P 005) A P S 30 d , B3 HO1 mRN A A ( P 005) COP D HO1 ; AP S HO1 COP D ! ; ; 1 ! R563 ! A ! 2010) Eff ect of astragalus polysaccharide on the expression of heme oxygenase1 in the lung tissue of rats with chronic obstructive pulmonary disease HAN K oulan , LI Ro ngliang , CHAN G T angxi , et a l . Depa tment of Medica l T echno lo gy , Yancheng Health P ofessional T echnolo gy Institute , Y ancheng 224005, CHI NA Abstract! Obj ective T o investig ate the effects of ast ag alus po lysaccha ide ( A P S) on the ex p ession of heme ox yg enase 1( H O1) in the lung t issue of ats with ch o nic obst uctive pulmo na y disease( CO PD) . Methods Six ty ats w e e andomly divided into 5 g oups of A ( COPD mo del) , B 1 ( t eated w ith 10 mg/ kg ) , B2( t eated w ith 20 mg/ kg ) , B3( t eated with 40 mg/ kg ) and C ( no mal co nt ol) . CO PD at model w as established by smoke inhalations and int at achea l instillat ion of lipo po lysaccha ide( L PS) . T he ex p ession levels of H O1 mR N A and p o tein in the lung tissues w e e espectively analyzed by RTP CR and Weste n blot befo e and afte A PS inte ventio n. Results T he ex p ession lev els of H O1 mRN A and p o tein in g oup A w e e hig he than tho se in g oup C afte 1, 15, 30 d of A PS administ atio n( P 005) . A fte 30 d, HO1 mRN A and p otein levels in g oup B3 we e sig nificantly highe than tho se in g oup A ( P 005) . Conclusion H O1 exp essio n inc eases in the lung tissue of CO PD at model. A PS can educe lung inju y by H O