复方甘草酸苷在猪肝细胞悬浮培养中的应用.pdfVIP

论 著 复方甘草酸苷在猪肝细胞悬浮培养中的应用① 蔡鸿宇 张素青 邵冰峰 周益龙 张--,5, (南通大学附属肿瘤医院肝胆外科南通市肿瘤医院肝胆外科 江苏南通 226361) 【摘要】目的 探讨复方甘草酸苷对体外悬浮培养肝细胞的保护作用。方法 原位胶原酶循环肝灌注法分离猪肝细胞，将分离所得猪 肝细胞分为2组，复方甘草酸苷组培养基 中含有800ug／mL的复方甘草酸苷，对照组为普通培养组，悬浮培养1周，观察形态和功能的变化。 结果 2组肝细胞培养12h后均形成多细胞球样聚集体 ，复方甘草酸酐培养组肝细胞成团更为明显；复方甘草酸苷培养组上清液ALB在培 养后24h3d、5d高于单纯肝细胞培养组(尸0．05)，在培养24h后，与培养后12h相比，复方甘草酸苷组AI开始升高，普通培养组ALB开始 降低，其后2组都开始升高，至7d时下降(尸0．05)；2组肝细胞培养上清液BUN含量随培养时间延长逐渐升高，但2组之问差异无统计学意 义 0．05)；2组肝细胞上清液中ALT含量逐渐下降，第3～7天复方甘草酸苷培养组组ALT含量低于单纯肝细胞培养组(尸0．05)。结论 复方甘草酸苷对体外悬浮培养的肝细胞具有保护作用。 【关键词】复方甘草酸苷 肝细胞培养 生物人工肝 【中图分类号】R979．1 【文献标识码 lA 【文章编号l1674—0742(20o9)12(a)--O013--03 Compound Glycyrrhizin in Suspension Culture ofPorcineHepatocytes [AbstractlObjective ToevaluatetheCompoundglycyrrhizininfuencesinsuspensioncultureofporcinehepatocytes．Methods Porcine hepatocyteswere isolated by in situ reciculating collagenase liverperfusion method．The porcinehepatocytes isolated werecultured in twoways．The hepatocyteswerecultured in 7 days．Morhological，vitality，albumin and activity ofhepatocytesgrowing in Group I(commonculturegroupco--culturedhepatoeytes)andGroupII(groupwithcompoundglyrrhizin)wereobservedrespectively．Results After 12h two groupsofhepatocyteswerecultured，the hepatocytesaggreated tobe hepatocyte spheres，Group IImoreobvious．After culture for 1， 3 ，and 5d，the contentofALB wassignificantly higher in compound glyrrhizin group than in common culturegroup． While on day 3， 5，and 7，the levelofALT wasmarkly lower．From 0．5dto 7d，therewasno significantdifferenceofthecontent of BUN between two goups．Conclusion Compound glycyrrhizin can recover and maintain thee functions of suspension cultured hepatocyes isolate by by in situ reciculating collagenase liver perfusion method． [Key Words】Compound glycyrrhizin；Hepatocyte culture；Bioartificialliver 急性肝衰竭是各种肝脏疾病特别是病毒性肝炎的主要死 因之