猪鸡致病菌β-内酰胺酶, 超广谱β-内酰胺酶检测与药敏试验[J].pdfVIP

中国农业科学 2005,38(2):399-404 Scientia Agricultura Sinica 猪鸡致病菌 β-内酰胺酶、超广谱β-内酰胺酶检测与药敏试验 胡功政，张春辉，梁 军，余 华，王升运，杨玉荣 （河南农业大学畜牧兽医学院，郑州 450002 ） 摘要: 对分离的猪鸡致病菌，分别进行了 ß －内酰胺酶、超广谱β-内酰胺酶(ESBLs)检测，并用试管二 倍稀释法测定了β－内酰胺环类抗生素或β－内酰胺环类抗生素与抑制剂舒巴坦联用对非产酶菌、产 ß －内 酰胺酶菌、产 ESBLs 菌的抗菌活性。结果表明，43 株分离菌的大多数可产生 ß －内酰胺酶，3 株鸡志贺氏菌为 ESBLs 阳性菌株。30株分离产酶菌均对阿莫西林、氨苄西林耐药（MIC≥32 µg/ml），16 株非产酶菌（标准菌株 及分离株）中的15株对阿莫西林、氨苄西林敏感，只有一株非产酶分离菌耐药。氨苄西林与舒巴坦以2∶1、4∶ 1配比联用时，其对分离产酶菌的MIC值分别降低10~40 倍、10~20倍。临床致病菌对三代头孢均敏感，与舒巴 坦以 2∶1、4∶1 配比联用时，三代头孢的 MIC 值不变。当头孢噻呋、头孢曲松与舒巴坦以 2∶1 配比联用时， 其对产 ESBLs 菌的MIC值降低2～4倍。 关键词: β-内酰胺酶；超广谱β-内酰胺酶；抑制剂；药敏试验 Detection of Beta-lactamase and Extended-spectrum Beta-lactamase and Antibiotic Susceptibility Test Analysis of Pathogens Isolated from Pig and Chicken HU Gong-zheng, ZHANG Chun-hui, LIANG Jun, YU Hua, WANG Sheng-yun, YANG Yu-rong (Faculty of Animal husbandary and Veterinary Science,Henan Agricultural University,Zhengzhou 450002 ) Abstract: The antibacterial activity of beta-lactam antibiotics or their combinations with inhibitor sulbactum against non-lactamase-producing strains, lactamase-producing and ESBLs-producing isolates was evaluated with two-fold dilution method after pathogens isolated from pigs and chickens were detected, respectively, for beta-lactamase and extended-spectrum beta-lactamases(ESBLs).The results revealed that most of the 43 clinically isolated strains could produce beta-lactamase and 3 strains of Shigella isolated from chicken samples produced ESBLs. Ａll of the 30 lactamase-producing strains isolated and only one of the 16 non-lactamase-producing strains were resistant to amoxicillin and ampicillin. MICs of ampicillin against lactamase-producing isol