人胎儿发育过程EGF、TGF-β1NGF、PCNA对睾丸组织细胞增殖的影响.pdf

中文 摘 要 免疫阳性细胞胞浆为桔黄色或棕黄色的颖粒状，且随着胎龄 的增长而减少，各胎龄组间均有显著性差异 (P0.00a 3.PCNA阳性细胞的表达:12周曲细精管索己经形成， 且连接成网，形态不规则，索内可见大量阳性细胞;16- 32周翠丸间质细胞、支持细胞、生殖细胞均有阳性表达，阳 J胜细胞核呈桔黄色或棕黄色。在16^20周PCNA免疫阳性 细胞高表达，而后随着胎龄的增长而减少 (P0.01). 结论:1.增殖细胞核抗原 (PCNA)免疫阳性细胞在人 胎儿不同胎龄皋丸组织细胞中均有表达，1620周为表达高 峰，而后随着胎龄的增长而减少;2.人胎儿翠丸组织不同发 育时期均可产生EGF,TGF-01,NGF,1620周为表达高 峰，而后随着胎龄的增长而减少，皋丸间质细胞是主要的内 分泌细胞，EGF,TGF-01,NGF可能通过自分泌或旁分泌 的作用在胚胎早期促进皋丸组织细胞的增殖、分化和成熟。 关键词:神经生长因子;表皮生长因子;转化生长因子- 01;增殖细胞核抗原;免疫组织化学;胎儿翠丸 英 文 摘 要 TheinfluenceofEGF,TGF-01NGFandPCNAon theproliferationofthegermcellsduringthe developmentofthefetustestis ABSTRACT Objective:ToobservetheexpressingfeaturesofPCNA、 EGF、NGF、 TGF-131ingerm cellsandstudytheir functionsduringthedevelopmentofthefetaltestes.Weexpect toprovidetheoreticalbasesandmorphologicaldataforthe stu勿ofmalereproductiveandphysiology. Methods:30fetaltesteswereused(theirfetalagewere12 weeksto32weeks)inthisstudyanddividedintosixgroups accordingtothefetalage.Thetestesweretakenoutandputinto 4%paraformaldehyde,fixed inthererfigeratoryfor24hours, washedbyPBSbuffer,embeddedinparaffineandsectioned continuouslyasusual.SPimmunocytochemistrytechnologyand lightmicroscopewereapplied.Thepositivecellnumberper areaineachsectionwasinputintothecomputer,andanalysed bySPSSstatisticalsoftware. Results:WefoundthatEGF.TGF-P1,NGFandPCNA expressedinvaryingdegreesindifferentperiodduringthe developmentofthefetaltestes. 1.TheexpressionofEGFandNGF:Thepositivecellswerenot foundin12thweek.From16thto32thweek,theywerefoundin Sertolicellsoccasionally,andinLeydigcellsalot,butnot 英 文 摘 要 foundingermcells.ThepeakexpressioninLeydigcellswas observedrfom16thto20thweek.Thepositiveproductionswere orangeorbrownandscatteredwithinthecytoplasm,andthe numberofpositivecellsdecreasedgraduallywiththeincreasing