乳酸菌拓扑异构酶+gyrA基因突变与耐喹诺酮类药物的相关性.pdfVIP

Association of Mutation Patterns in GyrA Genes and Quinolone Resistance Levels in Lactic Acid Bacteria Abstract 23 strains were isolated from koumiss in Xilin Gol in Inner Mongolia, which were studied by the experiments on some aspects，such as morphology, physiological and biochemical characteristics, molecular biology. The results showed that there were 4 strains of Enterococcus raffinosus, 3 strains of Enterococcus durans, 3 strains of Enterococcus dispar, 2 strains of Lactobacillus plantarum , 2 strains of Enterococcus villorum, 2 strains of Enterococcus gallinarum, 1 strain of Lactobacillus sake, 1 strain of Lactobacillus casei, 1 strain of Enterococcus pseudoavium , 4 strains were not lactic acid bacteria. The characteristics of physiology and biochemistry and the inhibitory test were studied, the good characteristics were found such as the ability to grow adequately in the condition of 6.5% NaCl and pH3.5, thermotolerance, the ability to ferment disaccharide and decimpose starch. The tested bacteria had different degrees of inhibition to Escherichia coli and Enteritidis bacillus, HZ1, HZ15, HZ24, HZ25, HZ26, HZ27, HZ28 and NN1 also had different degrees of inhibition to Staphylococcus aureus. Lactic acid bacteria using cylinder plate method and the liquid double dilution test to determine the antimicrobial resistance and MICs, The results showed that 23 strains had different degrees of resistance to Norfloxacin(NOR) and Ciprofloxacin (CIP), the rates were 100%, the maximum MICs were larger than 237.03 μg/mL. The sequences of quinolone resistance-determining region (QRDR) of gyrA were studied, and compared them with Eschericha coli through MegAlign, The results showed that the gyrA of HZ1, HZ15, HZ24, HZ25, NN, NN1, NN2, NN3