苦参联合左氧氟沙星对腹膜透析管大肠杆菌生物被膜与影响.docVIP

苦参联合左氧氟沙星对腹膜透析管大肠杆菌生物被膜的影响 梁俐 廖蕴华△ 邹龙涛 王成玉 陈一强 （广西医科大学第一附属医院肾内科 南宁 530021） 通讯作者：廖蕴华 联系电话0771-5303966 广西医科大学第一附属医院肾内科 530021 【摘要】目的：建立大肠杆菌(E.coli)生物被膜（BF）的体外模型，研究在体外苦参水煎液对BF的影响及对左氧氟沙星（LFX）的增效作用。方法：采用腹透液—腹膜透析管系统培养BF，建立体外BF模型，扫描电镜（SEM）观察BF变化。将苦参水煎液、LFX各药单独或联合作用于BF，连续稀释法进行存活菌菌落计数。结果：16 g／L的苦参水煎液即可抑制和破坏BF。与LFX联合应用后，BF内存活菌数均明显减少(P0．01) 。结论：苦参水煎液在体外可破坏E.coli已形成的BF，并可增强LFX对BF内E.coli的抗菌活性。 【关键词】大肠杆菌 ； 腹膜透析管 ；苦参 ；氧氟沙星 ln—Vitro Effect of Sophora flavescens Aqueous—Extracts Combination with Levofloxacin on Escherichia coli Biofilm LIANG Li，LIAO Yunhua，ZOU Longtao，WANG Chengyu,CHENG Yiqiang Department of Nephrology Medicine，First Affiliated Hospital ,Guangxi Medical University,Guangxi 530021, China Abstract Objective：To observe the inhibitory eflfect of sophora flavescens aqueous—extracts on biofilm of escherichia coli (E．coli)and the synergism of actericidal effect between sophora flavescens aqueous—extracts and levofloxacin (LFX)on biofflm of E．coli in vitro．Methods：Escherichia coli were cultivated in peritoneal dialysis solution –peritoneal dialysis cathete system to establish in vitro bacterial biofilm model，which was observed under scanning electronic microscope(SEM)．After the biofilm was treated by sophora flavescens aqueous—extracts，LFX and their combination，the number of viable bacteria was measured by serial dilutions．Results：Combined with sophora flavescens aqueous-extracts，LFX reduced the number of viable E．coli compared with LFX alone (P0．01) ．Conclusion：sophora flavescens aqueous—extracts can destruct the biofdm of E．coli and enhance antibacterial activities of LFX by increasing permeability of LFX into biofilms． Key words escherichia coli ；peritoneal dialysis cathete ；sophora flavescens ；ofloxacin 1引言 细菌生物被膜(biofilm，BF)是指细菌吸附于惰性物体如生物医学材料或机体黏膜表面后，分泌多糖基质将细菌体克隆聚集缠绕其中形成的膜样物【1】。由于细菌BF的存在，使被膜内的细菌能够逃逸抗生素的杀伤作用和机体免疫系统的清除，成为潜在的感染源，从而造成感染的反复发作【2】。大肠杆菌是导致腹膜炎的最常见G-菌【3】，主要原因是腹膜透析相关性腹膜炎，而腹透管则为细菌生物膜的生成提供了条件。如何抑制细菌BF的形成，尤其是对腹膜透析管BF的影响，目前国内尚无相关报道。本文主要