维生素E对Py17XL感染BALBc小鼠早期Th1应答的免疫调节作用.docVIP

维生素E对P.y17XL感染BALB/c小鼠早期Th1免疫调节作用 杨志蕃1 延娟2 刘军2 孙晓丹2 郑丽2 曹雅明2** （1.沈阳军区总医院肾内科，沈阳 邮编110016；2.中国医科大学基础医学院免疫学教研室，沈阳 邮编 110001） 【摘要目的 探讨维生素E（vitamin E, VE）对致死型约氏疟原虫（Plasmodium yoelii 17XL，P.y17XL）感染BALB/c小鼠Th1免疫应答的调节效应。 方法6-8周、雌性BALB/c小鼠，随机分为实验组和对照组，经腹腔接种1×106 P.yl7XL寄生的红细胞，实验组于感染前10d给予VE预处理。流式细胞术检测感染后第0d、3d和5d脾Tregs的百分含量；ELISA检测脾细胞培养上清中的IFN-(、IL-10的水平；Griess反应检测脾细胞培养上清中一氧化氮（NO）的含量。 结果与正常组相比（）补充VE组Tregs明显增多；（）补充VE组IFN-(和NO分泌减少，而IL-10的分泌水平增加。 结论 在P.yl7XL感染早期，VE预处理可显著抑制Th1免疫应答效应，从而进一步加剧P.y17XL感染BALB/c的死亡进程。关键词VE；致死型约氏疟原虫；Th1应答R382．31 【文献标识码】 A The immunoregulatory effect of Vitamin E on the Th1 immune responses during the early stage of Plasmodium yoelii 17XL infection in BALB/c mice YANG Zhifan1,YAN Juan2, LIU Jun2, SUN Xiaodan2, ZHENG Li2, CAO Ya-ming2 (1. Department of Nephrology , Shenyang General Military Hospital, Shenyang 110016, China; 2. Department of Immunology, College of Basic Medical Sciences, China Medical University, Shenyang 110001, China) 【Abstract】 Objective To investigate the Th1 immunoregulatory effect of VitaminE(VE) in BALB/c mice infected with Plasmodium yoelii 17XL（P.y17XL）. Method Female 6–8-week-old BALB/c mice were randomly divided into two groups. The mice in VE group were orally administered VE once a day for successive 10 days before P.y17XL infection. Flow cytometry was introduced to detect the percentage of CD4+CD25+regulatory T Cells (Tregs). The level of IFN-γ, IL-10 and NO in the supernatant of splenocytes culture were detected by ELISA and Griess reaction, respectively. Results (1) The percentage of Tregs was obviously higher in VE group. (2) IFN-γ and NO was reduced in VE group, while IL-10 was increased. Conclusions Pretreatment with VE significantly suppressed Th1 immune response during the early stage of P.y17XL infection in BALB/c mice; therefore, the course of infection was further aggravated. 【Key words】VE; Plasmodium yoelii 17XL; Th1 immune response; immunoregulation *【基金项目】高等学校博士学科点专项科研基金（20112104110018） **【通讯作者】曹雅明，