模拟失重增强大鼠脑动脉血管平滑肌细胞大电导钙激活钾通道功能.pdf

Acta Physiologica Sinica, August 25, 2005, 57 (4): 439-445 439 * 710032 : (vascular smooth muscle cells, VSMCs) (large conductance calcium-activated potassium channels, BKCa channels) VSMCs ([Ca2+] ); BK i Ca 1 VSMCs [Ca2+] (P0.05); BK (Po) i Ca (To)(P0.05)(Tc)1 VSMCs BK [Ca2+] VSMCs Ca i ; C a 2 + R852.22 Enhanced BKCa single-channel activities in cerebrovascular smooth muscle cells of simulated microgravity rats * XIE Man-Jiang, ZHANG Li-Fan , MA Jin, CHENG Hong-Wei Department of Aerospace Physiology, Fourth Military Medical University, Xi an 710032, China Abstract: The aim of the present study was to investigate the changes in single-channel currents of large conductance calcium-activated potassium channels (BKCa channels) in cerebral vascular smooth muscle cells (VSMCs) of rats after 1-week simulated microgravity. Sprague-Dawley rats were subjected to tail-suspension (SUS) to simulate cardiovascular deconditioning due to microgravity. Cytosolic 2+ calcium ([Ca ] ) was examined by laser-scanning confocal microscopy with calcium-sensitive-dye Fluo-3/AM as fluorescent probe. i Single-channel currents of BKCa channels were measured with cell-attached membrane patches bathed in symmetrical high potassium 2+ solution. The [Ca ] level was significantly higher in cerebrovas