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Acta Physiologica Sinica, August 25, 2005, 57 (4): 439-445 439
*
710032
: (vascular smooth muscle cells, VSMCs)
(large conductance calcium-activated potassium channels, BKCa channels)
VSMCs ([Ca2+] ); BK
i Ca
1 VSMCs [Ca2+] (P0.05); BK (Po)
i Ca
(To)(P0.05)(Tc)1 VSMCs
BK [Ca2+] VSMCs
Ca i
; C a 2 +
R852.22
Enhanced BKCa single-channel activities in cerebrovascular smooth muscle cells
of simulated microgravity rats
*
XIE Man-Jiang, ZHANG Li-Fan , MA Jin, CHENG Hong-Wei
Department of Aerospace Physiology, Fourth Military Medical University, Xi an 710032, China
Abstract: The aim of the present study was to investigate the changes in single-channel currents of large conductance calcium-activated
potassium channels (BKCa channels) in cerebral vascular smooth muscle cells (VSMCs) of rats after 1-week simulated microgravity.
Sprague-Dawley rats were subjected to tail-suspension (SUS) to simulate cardiovascular deconditioning due to microgravity. Cytosolic
2+
calcium ([Ca ] ) was examined by laser-scanning confocal microscopy with calcium-sensitive-dye Fluo-3/AM as fluorescent probe.
i
Single-channel currents of BKCa channels were measured with cell-attached membrane patches bathed in symmetrical high potassium
2+
solution. The [Ca ] level was significantly higher in cerebrovas
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