香蕉植株内生细菌群落多态性的研究.docVIP

香蕉植株内生细菌群落多态性研究 蓝江林1，刘波1*，焦会民2，朱育菁1，苏明星1，史怀1(， （1.福建省农业科学院农业生物资源研究所，福建 福州 350003；2.福建农林大学，福建 福州 350002） 摘 要：【目的】了解香蕉植株在不同生长状态下内生细菌的群落多样性。【方法】采用平板分离纯化香蕉健株和病株内生细菌，利用细菌脂肪酸鉴定技术进行鉴定。【结果】共获得内生细菌21属24种，香蕉健株共分离获得9株内生细菌，6种细菌分布在根部，茎部分离到2种，叶部分离到8种细菌。香蕉病株共分离获得17株内生细菌，分属15个属，从根部分离到3种细菌，茎部分离到11种，叶部共分离到6种。就内生细菌的含量来看，香蕉健株根部的内生细菌含量最高，达5.195×106cfu/g，下部叶片内生细菌的含量最低，仅为0.30×102cfu/g。香蕉病株茎部内生细菌的数量显著高于其他部位，达1.05×107cfu/g。【结论】香蕉植株在不同生长状态下内生细菌的种类和数量均存在多样性。 关键词：香蕉；内生细菌；群落多态性 中图分类号： 文献标识码： 文章编号： Community and diversity of endophytic bacteria inside Musa plants LAN Jiang-lin1，LIU Bo1*，JIAO Hui-min2，ZHU Yu-jing1，SU Ming-xing1，SHI Huai1 （1.Agricultural Bioresource Research Institute，Fujian Academy of Agricultural Sciences, Fuzhou 350003；2.College of Plant Protection，Fujian Agriculture and Forestry University，Fuzhou 350002） Abstract:【Objective】In order to study the community structure of endophytic bacteria in Musa paradisiacal.【Method】The healthy and disease infested Musa paradisiaca plants were collected from Zhangzhou in Fujian Province, PR China. The endophytic bacteria were isolated using the culture method and identified by Microorganism Identification System （MIDI system produced by USA）.【Result】The results showed that a total of 24 microbial species identified belonging to 21 genera of bacteria were colonized detected in Musa plants.There were 9 microbial species belonging to 9 genera were found inside the healthy plant, of which,6 species were found inside the roots with the high amount of 5.96×105CFU·g-1, 2 species were found inside stems and 8 species of bacteria were found inside leaves.There were 17 microbial species belonging to 15 genera were displayed in the diseased infested plant, 11 species of bacteria were found inside the stems with the high amount of 1.05×107cfu/g, 3 species were found inside roots and 6 species of bacteria were found inside leaves .【Conclusion】There are significant polymorphism among community structure of endophytic bacteria and