基质金属蛋白酶8 在食管鳞癌中的表达及其临床意义.docVIP

基质金属蛋白酶8在食管鳞癌中的表达及其临床意义 王冬梅1,2，郭 洪1，陈雪丹1，官兴颖1，刘 丹1，白 云（400038 重庆，第三军医大学基础部遗传教研室1；430010 武汉，解放军161医院检验科2） [摘要] 目的 探讨食管鳞状细胞癌中基质金属蛋白酶8（MMP-8）的表达及其与肿瘤浸润、转移的关系。方法 实时荧光定量PCR（Real-time PCR）方法检测67例食管鳞癌组织和27例远癌组织中MMP-8 mRNA的表达；Western blot法检测其中15例食管鳞癌组织及其配对的15例远癌组织中MMP-8蛋白的表达，统计分析MMP-8表达水平与食管鳞癌的临床分期以及浸润转移的关系。结果 （1）MMP-8在食管鳞癌组织和远癌组织中均有不同程度的表达，在癌组织的表达水平显著低于远癌组织（mRNA表达水平 P=0.003，蛋白表达水平 P=0.031）。（2）T1期、T2期、T3期以及T4期食管鳞癌组织中MMP-8 mRNA表达存在显著差异（P=0.002），MMP-8表达随着肿瘤浸润程度的加深而升高；MMP-8蛋白表达量在T1～T4期中也呈增加的趋势。（3）MMP-8 mRNA和蛋白表达水平与食管鳞癌的淋巴结转移无显著关联。结论 食管鳞癌组织中MMP-8的表达较远癌组织降低，其表达水平又与食管鳞癌的浸润程度正相关，可能反映了MMP-8在食管鳞癌发生、发展过程中的不同作用机制。 [关键词] 食管鳞状细胞癌；基质金属蛋白酶8；浸润；转移；表达 [中图法分类号] R394.3; R730. 2; R735.1 [文献标志码] A Expression of Matrix Metalloproteinase 8 in Esophageal Squamous Cell Carcinoma Wang Dongmei1,2， Guo Hong1， Chen Xuedan1， Guan Xingying1， Liu Dan1， Bai Yun1（Department of Medical Genetics, College of Basic Medical Science, Third Military Medical University1, Chongqing，400038; Department of Laboratory Medical, 161 Hospital of PLA2, Wuhan 430010） Expression and Clinical Significance of Matrix Metalloproteinase 8 in Esophageal Squamous Cell Carcinoma [Abstract] Objective Matrix Metalloproteinase 8 (MMP-8) plays multiple roles in different cancers or different stage in cancer development. However, there is no report about MMP-8 expression and role in esophageal squamous cell carcinoma (ESCC) so far. The aim of this study is to determine the expression of MMP-8 in ESCC and explore the correlations between the expression of MMP-8 and invasion and metastasis of ESCC. Methods We detected MMP-8 mRNA expression in 67 ESCC tissues and 27 normal tissues with Real-time PCR and also detected its protein expression in 15 ESCC tissues and 15 matched normal tissues with Western blot, then explored the association of MMP-8 expression with clinical characteristics including invasion and metastasis. Results The mRNA and protein expression levels of MMP-8 were markedly lower in ESCC tissues than that in t