嗜热L_乳酸高产菌株的选育.pdfVIP

·1178 · CHEMICAL INDUSTRY AND ENGINEERING PROGRESS 2006 25 10 L- 210009 89 3 5055 L-S-18S-32 S-443 100 g/L 72 h 75.0 g/L 81.9 g/L 86.5 g/L96.3%96.6% 97.7%S-44 Y-4 200 g/L 178.7 g/L89.4 Q 93.33 A 1000 6613 2006 10 1178 06 Breeding of efficient L-lactic acid producing thermophiles XU Tingting BAI Zhongzhong HE Bingfang (College of Life Science and Pharmaccutical EngineeringNanjing University of Technology Nanjing 210009 Jiangsu China) Abstract: L-lactic acid is the most useful organic acid. In this study89 strains producing lactic acid were isolated from soil silage hot spring water and surrounding soil. The concentration and enantiomeric purity of lactic acid produced by these strains were determined by HPLC. Based on the ability of the strains to produce L-lactic acidthree effective strains S-18 S-32 and S-44 which grew at 50 and 55 were selected. After 72 h of fermentation75.0 g/L81.9 g/L and 86.5 g/L of L-lactic acid were produced from 100 g/L glucose by the three strainsrespectively. The enantiomeric purity of the products was 96.3% 96.6% and 97.7%respectively. Strain S-44 was mutated by the N+ ion beam implantationand more efficient mutants were obtained. By using the mutant strain Y-4178.7 g/L of L-lactic acid was produced with the molar yields of 178.7% to the substrate glucose. Key words: lactic acid screeningion beam implantation (lactic acid)α- L- [1] [45] [6]