大鼠单一肌梭的电生理特征.pdfVIP

生理学报Acta Physiologica Sinica , June 25, 2011, 63(3): 281–285 281 实验技术 大鼠单一肌梭的电生理特征 * 赵雪红，樊小力 ，宋新爱，石磊 西安交通大学医学院生理与病理生理学系，西安 710061 摘 要：本研究旨在观察大鼠单一肌梭的电生理特征。从大鼠比目鱼肌中分离单一肌梭，用空气隔绝法观察大鼠单一肌梭感 觉末梢在不同液体环境中的放电活动。结果显示：在基础生理盐水溶液中，大鼠单一肌梭的自发放电频率很低，平均(51.78 ± 25.63) impulses/1 000 s (n = 13) ；在加有适量氨基酸的生理盐水溶液中，其自发放电频率明显增多，每1 000 s记录到的动作 200~1 000 [ (687.62 ± 312.56) impulses/1 000 s, n = 17] 电位数为 个平均 ，而且能维持较长时间。大鼠单一肌梭的电活动除了典 abortive spikes 型的动作电位之外，还记录到了大量的阈下去极化电位 “ ”。结果表明，生理盐水溶液中添加适量氨基酸能 够很好地保持大鼠单一肌梭的活性。本研究为深入了解大鼠肌梭的功能奠定了方法学基础。 关键词：肌梭；电生理；空气隔绝法 中图分类号：R852.2 Electrophysiological characteristics of the isolated muscle spindle in rats * ZHAO Xue-Hong, FAN Xiao-Li , SONG Xin-Ai, SHI Lei Department of Physiology and Pathophysiology, Medical School, Xi’an JiaoTong University, Xi’an 710061, China Abstract: The aim of this study was to observe the electrophysiological characteristics of the isolated rat muscle spindle. The muscle spindle was isolated from rat soleus and the afferent discharge of the isolated muscle spindle was recorded by air-gap technique. In the basic physiological salt solution, the spontaneous impulses of muscle spindle were at a lower level with irregular intervals. The mean frequency of afferents was (51.78 ± 25.63) impulses/1 000 s (n = 13). The muscle spindle afferents were significantly increased and maintained over time by the addition of certain amino acids during the observation. The number of the action potential recorded per 1 000 s was 200–1 000 [mean: (687.62 ± 312.56) impulses/1 000 s, n = 17]. In addition to the typical propagated action potential, a large number of abortive spikes were observed. The results indicate that the activities of isolated muscle spindles in rats