不同膀胱病理状态下庆大霉素膀胱冲洗的安全性考察 期刊论文.docVIP

不同膀胱病理状态下庆大霉素膀胱冲洗的安全性考察 陈旭1 刘萍1 高媛1 许冬梅2 孙淑娟2 （1 山东大学药学院，山东 济南 250012；2 山东省千佛山医院，250014） 【摘要】 目的：考察庆大霉素（GTM）膀胱冲洗在细菌性膀胱炎及出血性膀胱炎下的安全性。方法：收集接受GTM膀胱冲洗患者给药后30 min的血液（80 mg，2次/d），以荧光偏振免疫法（FPIA法）测定血药浓度；12只Wistar大鼠随机等分为对照组和模型组，模型组腹腔注射环磷酰胺140 mg/kg构建出血性膀胱炎模型，并以苏木精-伊红染色法（hematoxylin-eosin staining，HE染色法）观察造模结果。造模24 h后，两组大鼠分别给予GTM 8.4 mg/kg膀胱内注射，FPIA法测定给药30 min后血药浓度。结果：共有8例次患者进入实验，监测GTM平均血药浓度为0.234 μg/mL（0.14 ~ 0.40 μg/mL）；模型组大鼠在接受GTM膀胱内注射后平均血药浓度为（7.53±1.32）μg/mL，与对照组（0.63±0.12） μg/mL相比有显著统计学差异（P 0.01）。结论：GTM膀胱冲洗对于细菌性膀胱炎是安全的，但应避免用于出血性膀胱炎患者。 【关键词】 庆大霉素；膀胱冲洗；细菌性膀胱炎；出血性膀胱炎 doi:10.3969/j.issn.1672-5433.2012.05.006 The Safety of Gentamicin Bladder Irrigations in Different Pathological Conditions Chen Xu1， Liu Ping1，Gao Yuan1， Xu Dongmei2， Sun Shujuan2（1 School of Pharmacy of Shandong University，Shandong Jinan 250012，China；2 Qianfoshan Hospital of Shandong Province，250014） ABSTRACT Objective：To assess the safety of gentamicin bladder irrigations in patients with bacterial cystitis or hemorrhagic cystitis. Methods：Blood was collected from the patients who had received gentamicin bladder irrigations （80 mg of gentamicin， twice a day）. FPIA method was used to test the gentamicin concentration. Twelve Wistar Rats were used and divided randomly into model and control groups. The hemorrhagic cystitis rat model was set up by intraperitoneal injection with 140 mg/kg CTX and justified by HE staining. 24 h later， the rats of the two groups were treated with 8.4 mg/kg gentamicin bladder irrigations， and the concentration of gentamicin was tested by FPIA method. Results：Eight patients were enrolled in this study and the tested concentrations of gentamicin were ranged from 0.14 to 0.40 μg/mL with a median value of 0.234 μg/mL. In the animal study， the tested median concentration of gentamicin in hemorrhagic cystitis rat model was （7.53±1.32） μg/mL， much higher than the control group （0.63±0.12）μg/mL （P 0.01）. Conclusion： Gentamicin bladder irrigations were proved safety in use in the patie